<mxfile host="app.diagrams.net"><diagram name="roadmap"><mxGraphModel dx="1200" dy="800" grid="1" gridSize="10" guides="1" tooltips="1" connect="1" arrows="1" fold="1" page="1" pageScale="1" pageWidth="1400" pageHeight="900" math="0" shadow="0">
  <root>
    <mxCell id="0" />
    <mxCell id="1" parent="0" />
    
    <mxCell id="node1" vertex="1" parent="1" value="研究问题：甜菊糖苷含量低、比例不佳" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#E1D5E7;strokeColor=#8B5E9E;fontSize=12;fontStyle=1;verticalAlign=middle;align=center;">
      <mxGeometry x="488" y="40" width="264" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node2" vertex="1" parent="1" value="策略：构建过表达与anti-miRNA载体，共表达anti-miR319g和miRStv_11" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#DEEBF7;strokeColor=#5B9BD5;fontSize=12;fontStyle=1;verticalAlign=middle;align=center;">
      <mxGeometry x="450" y="162" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node3" vertex="1" parent="1" value="关键改造①：anti-miR319g敲低miR319g，解除对KO、KS、UGT85C2抑制" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#FCE4D6;strokeColor=#ED7D31;fontSize=12;verticalAlign=middle;align=center;">
      <mxGeometry x="40" y="284" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node4" vertex="1" parent="1" value="关键改造②：miRStv_11过表达结合KAH启动子，正调控KAH表达" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#FCE4D6;strokeColor=#ED7D31;fontSize=12;verticalAlign=middle;align=center;">
      <mxGeometry x="450" y="284" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node5" vertex="1" parent="1" value="关键改造③：共表达anti-miR319g和miRStv_11协同激活关键酶基因" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#FFF2CC;strokeColor=#BF8F00;fontSize=12;verticalAlign=middle;align=center;">
      <mxGeometry x="245" y="406" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node6" vertex="1" parent="1" value="结果：靶基因KO(↑11x)、KS(↑6x)、UGT85C2(↑7x)、KAH(↑12x)" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#E2EFDA;strokeColor=#70AD47;fontSize=12;verticalAlign=middle;align=center;">
      <mxGeometry x="860" y="284" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node7" vertex="1" parent="1" value="结果：甜菊苷143.9 mg/g(↑24.5%)，Reb A 69.0 mg/g(↑51%)" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#E2EFDA;strokeColor=#70AD47;fontSize=12;verticalAlign=middle;align=center;">
      <mxGeometry x="655" y="406" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node8" vertex="1" parent="1" value="结果：Reb A/Stevioside比例0.39→0.47；miRStv_11-KAH启动子结合ACE=-937.59" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#E2EFDA;strokeColor=#70AD47;fontSize=12;verticalAlign=middle;align=center;">
      <mxGeometry x="450" y="528" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node9" vertex="1" parent="1" value="结论：miR319g负调控、miRStv_11正调控甜菊糖苷合成；共表达显著改良甜菊糖苷品质" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#F8CBAD;strokeColor=#C55A11;fontSize=12;fontStyle=1;verticalAlign=middle;align=center;">
      <mxGeometry x="450" y="650" width="340" height="64" as="geometry" />
    </mxCell>
    
    <mxCell id="edge1" edge="1" parent="1" source="node1" target="node2" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#8B5E9E;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge2" edge="1" parent="1" source="node2" target="node3" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#5B9BD5;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge3" edge="1" parent="1" source="node2" target="node4" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#5B9BD5;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge4" edge="1" parent="1" source="node3" target="node5" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#6C3483;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge5" edge="1" parent="1" source="node4" target="node5" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#6C3483;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge6" edge="1" parent="1" source="node2" target="node6" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#5B9BD5;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge7" edge="1" parent="1" source="node5" target="node6" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0;exitY=0.5;exitDx=0;exitDy=0;entryX=0;entryY=0.5;entryDx=0;entryDy=0;dashed=1;dashed=1;strokeColor=#BF8F00;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge8" edge="1" parent="1" source="node6" target="node7" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#70AD47;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge9" edge="1" parent="1" source="node7" target="node8" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#70AD47;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge10" edge="1" parent="1" source="node8" target="node9" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#C55A11;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
  </root>
</mxGraphModel></diagram></mxfile>