<mxfile host="app.diagrams.net"><diagram name="roadmap"><mxGraphModel dx="1422" dy="794" grid="1" gridSize="10" guides="1" tooltips="1" connect="1" arrows="1" fold="1" page="1" pageScale="1" pageWidth="1169" pageHeight="826" math="0" shadow="0">
  <root>
    <mxCell id="0" />
    <mxCell id="1" parent="0" />
    <mxCell id="node1" value="问题：杜氏盐藻富含ALA但EPA含量低" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#dae8fc;strokeColor=#6c8ebf;fontSize=13;fontStyle=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="242" y="40" width="250" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node2" value="策略：过表达TpFADS6并优化培养条件" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#d5e8d4;strokeColor=#82b366;fontSize=13;fontStyle=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="238" y="162" width="257" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node3" value="关键改造1：构建Ds-TpFADS6-8藻株" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#fff2cc;strokeColor=#d6b656;fontSize=13;fontStyle=0;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="40" y="284" width="243" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node4" value="关键改造2：添加500mg/L肌醇强化" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#fff2cc;strokeColor=#d6b656;fontSize=13;fontStyle=0;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="78" y="406" width="236" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node5" value="关键改造3：12%CO2+葡萄糖/KNO3" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#fff2cc;strokeColor=#d6b656;fontSize=13;fontStyle=0;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="74" y="528" width="229" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node6" value="关键改造4：8°C低温+4g/L紫苏籽粕" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#fff2cc;strokeColor=#d6b656;fontSize=13;fontStyle=0;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="242" y="650" width="250" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node7" value="结果：EPA产量达554.3mg/L（343.8倍）" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#e1d5e7;strokeColor=#9673a6;fontSize=13;fontStyle=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="384" y="406" width="271" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node8" value="结论：杜氏盐藻可作为EPA大规模生产宿主" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#f8cecc;strokeColor=#b85450;fontSize=13;fontStyle=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="374" y="528" width="285" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node9" value="关键改造5：验证TpFADS6高活性（LA:70.4% ALA:89%）" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#fff2cc;strokeColor=#d6b656;fontSize=13;fontStyle=0;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="353" y="284" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="edge1" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#6c8ebf;" edge="1" parent="1" source="node1" target="node2">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge2" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#82b366;" edge="1" parent="1" source="node2" target="node3">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge3" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#d6b656;" edge="1" parent="1" source="node3" target="node4">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge4" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#d6b656;" edge="1" parent="1" source="node4" target="node5">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge5" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#d6b656;" edge="1" parent="1" source="node5" target="node6">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge6" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0;exitY=0.5;exitDx=0;exitDy=0;entryX=0;entryY=0.5;entryDx=0;entryDy=0;dashed=1;dashed=1;strokeColor=#9673a6;" edge="1" parent="1" source="node6" target="node7">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge7" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#b85450;" edge="1" parent="1" source="node7" target="node8">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge8" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#82b366;dashed=1;" edge="1" parent="1" source="node2" target="node9">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge9" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#d6b656;dashed=1;" edge="1" parent="1" source="node9" target="node7">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
  </root>
</mxGraphModel></diagram></mxfile>