<mxfile host="app.diagrams.net"><diagram name="roadmap"><mxGraphModel dx="1200" dy="800" grid="1" gridSize="10" guides="1" tooltips="1" connect="1" arrows="1" fold="1" page="1" pageScale="1" pageWidth="1400" pageHeight="900" math="0" shadow="0">
  <root>
    <mxCell id="0" />
    <mxCell id="1" parent="0" />
    <mxCell id="node1" value="问题：大肠杆菌异源蛋白易形成包涵体" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#dae8fc;strokeColor=#6c8ebf;fontSize=13;fontStyle=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="461" y="40" width="264" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node2" value="策略：开发CBM66新型融合标签" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#d5e8d4;strokeColor=#82b366;fontSize=13;fontStyle=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="312" y="162" width="215" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node3" value="改造：CBM66融合靶蛋白N端" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#fff2cc;strokeColor=#d6b656;fontSize=13;fontStyle=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="164" y="284" width="210" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node4" value="验证：七种难表达蛋白均获得可溶性表达" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#f8cecc;strokeColor=#b85450;fontSize=13;fontStyle=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="596" y="162" width="278" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node5" value="对比：CBM66 vs MBP/GST标签" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#f8cecc;strokeColor=#b85450;fontSize=13;fontStyle=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="444" y="284" width="210" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node6" value="成果：PETase可溶产量达370 mg/L（商业标签3.7倍）" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#e1d5e7;strokeColor=#9673a6;fontSize=13;fontStyle=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="40" y="406" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node7" value="纯化：levan-琼脂糖亲和层析，回收率88.6%" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#e1d5e7;strokeColor=#9673a6;fontSize=13;fontStyle=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="724" y="284" width="299" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node8" value="活性：融合蛋白保持天然活性（97.6%~97.9%）" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#e1d5e7;strokeColor=#9673a6;fontSize=13;fontStyle=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="450" y="406" width="313" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node9" value="结论：CBM66为高价值蛋白经济生产提供新工具" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#ffe6cc;strokeColor=#d79b00;fontSize=13;fontStyle=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="833" y="406" width="313" height="64" as="geometry" />
    </mxCell>
    <mxCell id="edge1" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;fontSize=12;" edge="1" parent="1" source="node1" target="node2">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge2" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;fontSize=12;" edge="1" parent="1" source="node2" target="node3">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge3" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;fontSize=12;" edge="1" parent="1" source="node3" target="node6">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge4" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;fontSize=12;" edge="1" parent="1" source="node1" target="node4">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge5" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;fontSize=12;" edge="1" parent="1" source="node2" target="node5">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge6" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;fontSize=12;" edge="1" parent="1" source="node4" target="node7">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge7" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;fontSize=12;" edge="1" parent="1" source="node5" target="node8">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge8" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0;exitY=0.5;exitDx=0;exitDy=0;entryX=0;entryY=0.5;entryDx=0;entryDy=0;dashed=1;dashed=1;dashed=1;fontSize=12;" edge="1" parent="1" source="node6" target="node7">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge9" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;fontSize=12;" edge="1" parent="1" source="node7" target="node8">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge10" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;fontSize=12;" edge="1" parent="1" source="node7" target="node9">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge11" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=1;exitY=0.5;exitDx=0;exitDy=0;entryX=0;entryY=0.5;entryDx=0;entryDy=0;fontSize=12;" edge="1" parent="1" source="node8" target="node9">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
  </root>
</mxGraphModel></diagram></mxfile>