<mxfile host="app.diagrams.net"><diagram name="roadmap"><mxGraphModel dx="1200" dy="800" grid="1" gridSize="10" guides="1" tooltips="1" connect="1" arrows="1" fold="1" page="1" pageScale="1" pageWidth="1100" pageHeight="850" math="0" shadow="0">
  <root>
    <mxCell id="0" />
    <mxCell id="1" parent="0" />
    
    
    <mxCell id="node1" value="问题：丙酮酸脱羧造成碳损失、产物收率低" vertex="1" parent="1" style="rounded=1;whiteSpace=wrap;html=1;verticalAlign=middle;align=center;">
      <mxGeometry x="218" y="40" width="292" height="64" as="geometry" />
    </mxCell>
    
    
    <mxCell id="node2" value="策略：引入Xfpk支路，碳守恒替代脱羧" vertex="1" parent="1" style="rounded=1;whiteSpace=wrap;html=1;verticalAlign=middle;align=center;">
      <mxGeometry x="232" y="162" width="264" height="64" as="geometry" />
    </mxCell>
    
    
    <mxCell id="node3" value="改造①：筛选高活性Xfpk酶（B. breve）" vertex="1" parent="1" style="rounded=1;whiteSpace=wrap;html=1;verticalAlign=middle;align=center;">
      <mxGeometry x="224" y="284" width="278" height="64" as="geometry" />
    </mxCell>
    
    
    <mxCell id="node4" value="改造②：甘油途径敲除glpR+过表达xfpk" vertex="1" parent="1" style="rounded=1;whiteSpace=wrap;html=1;verticalAlign=middle;align=center;">
      <mxGeometry x="412" y="406" width="271" height="64" as="geometry" />
    </mxCell>
    
    
    <mxCell id="node5" value="改造③：木糖途径敲除gcd+整合xylABE-xfpk" vertex="1" parent="1" style="rounded=1;whiteSpace=wrap;html=1;verticalAlign=middle;align=center;">
      <mxGeometry x="44" y="406" width="299" height="64" as="geometry" />
    </mxCell>
    
    
    <mxCell id="node6" value="结果：甘油生长+44.3%，木糖+167%" vertex="1" parent="1" style="rounded=1;whiteSpace=wrap;html=1;verticalAlign=middle;align=center;">
      <mxGeometry x="444" y="528" width="243" height="64" as="geometry" />
    </mxCell>
    
    
    <mxCell id="node7" value="结果：黄酮+38.5%~49.4%，甲羟戊酸+25.9%~48.7%" vertex="1" parent="1" style="rounded=1;whiteSpace=wrap;html=1;verticalAlign=middle;align=center;">
      <mxGeometry x="40" y="528" width="334" height="64" as="geometry" />
    </mxCell>
    
    
    <mxCell id="node8" value="结论：碳保守提高生长和产物收率，奠定NOG/GATCHYC基础" vertex="1" parent="1" style="rounded=1;whiteSpace=wrap;html=1;verticalAlign=middle;align=center;">
      <mxGeometry x="194" y="650" width="340" height="64" as="geometry" />
    </mxCell>
    
    
    <mxCell id="edge1" edge="1" parent="1" source="node1" target="node2" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    
    
    <mxCell id="edge2" edge="1" parent="1" source="node2" target="node3" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    
    
    <mxCell id="edge3" edge="1" parent="1" source="node3" target="node5" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    
    
    <mxCell id="edge4" edge="1" parent="1" source="node3" target="node4" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    
    
    <mxCell id="edge5" edge="1" parent="1" source="node4" target="node6" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    
    
    <mxCell id="edge6" edge="1" parent="1" source="node5" target="node7" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    
    
    <mxCell id="edge7" edge="1" parent="1" source="node6" target="node8" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    
    
    <mxCell id="edge8" edge="1" parent="1" source="node7" target="node8" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
  </root>
</mxGraphModel></diagram></mxfile>