<mxfile host="app.diagrams.net"><diagram name="roadmap"><mxGraphModel>
  <root>
    <mxCell id="0" />
    <mxCell id="1" parent="0" />
    <mxCell id="node1" value="研究问题：传统序列相似性方法难以挖掘细菌萜烯合酶，缺乏无序列依赖筛选体系" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#D9EAD3;strokeColor=#82B366;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="40" y="40" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node2" value="策略：构建基于前体毒性的功能宏基因组筛选系统" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#D9EAD3;strokeColor=#82B366;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="43" y="162" width="334" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node3" value="关键改造1：基因组精简LowMut宿主降低自发突变假阳性" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#D9EAD3;strokeColor=#82B366;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="40" y="284" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node4" value="关键改造2：优化筛选条件（8mM甲羟戊酸+0.5mM IPTG）与二次筛选流程" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#D9EAD3;strokeColor=#82B366;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="40" y="406" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node5" value="结果1：从人粪便宏基因组文库筛选到10个候选萜烯合酶" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#F8CECD;strokeColor=#B85450;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="40" y="528" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node6" value="结果2：发现新型β-法尼烯合酶TS10F1（与已知酶无序列相似性）" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#F8CECD;strokeColor=#B85450;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="40" y="650" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node7" value="结果3：酿酒酵母中实现β-法尼烯发酵生产（最高119.74mg/L）" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#F8CECD;strokeColor=#B85450;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="40" y="772" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node8" value="结论：建立无序列依赖的萜烯合酶挖掘平台，为萜烯微生物合成提供新酶资源" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#CFE2F3;strokeColor=#6D9EEB;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="40" y="894" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="edge1" edge="1" parent="1" source="node1" target="node2" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;"><mxGeometry relative="1" as="geometry" /></mxCell>
    <mxCell id="edge2" edge="1" parent="1" source="node2" target="node3" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;"><mxGeometry relative="1" as="geometry" /></mxCell>
    <mxCell id="edge3" edge="1" parent="1" source="node3" target="node4" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;"><mxGeometry relative="1" as="geometry" /></mxCell>
    <mxCell id="edge4" edge="1" parent="1" source="node4" target="node5" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;"><mxGeometry relative="1" as="geometry" /></mxCell>
    <mxCell id="edge5" edge="1" parent="1" source="node5" target="node6" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;"><mxGeometry relative="1" as="geometry" /></mxCell>
    <mxCell id="edge6" edge="1" parent="1" source="node6" target="node7" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;"><mxGeometry relative="1" as="geometry" /></mxCell>
    <mxCell id="edge7" edge="1" parent="1" source="node7" target="node8" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;"><mxGeometry relative="1" as="geometry" /></mxCell>
  </root>
</mxGraphModel></diagram></mxfile>