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    <mxCell id="node2" value="策略：对引入E.coli木糖基因的ZM4进行80天30次连续传代适应性进化，筛选出A3菌株" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#d4e6f1;strokeColor=#57908c;fontSize=11;fontStyle=0;verticalAlign=middle;align=center;" vertex="1" parent="1">
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    <mxCell id="node3" value="关键改造：PpdC启动子-23bp处G→A突变（xylA表达增强，木糖异构酶活性提高4-8倍）" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#f8d7da;strokeColor=#c0504d;fontSize=11;fontStyle=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
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    <mxCell id="node4" value="结果1：5%葡萄糖-5%木糖混合发酵——木糖比消耗速率1.8 g/g/h（A1:0.45），乙醇得率96.6%" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#e2efda;strokeColor=#82b366;fontSize=11;fontStyle=0;verticalAlign=middle;align=center;" vertex="1" parent="1">
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    <mxCell id="node5" value="结果2：10%木糖为唯一碳源——46h耗尽木糖，乙醇4.31%，比消耗速率3.44 g/g/h（A1仅用1%）" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#e2efda;strokeColor=#82b366;fontSize=11;fontStyle=0;verticalAlign=middle;align=center;" vertex="1" parent="1">
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    <mxCell id="node6" value="结果3：两次补料混合发酵（总糖20%）——共产乙醇9%，得率93.4%（当时Zymomonas最高报道）" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#e2efda;strokeColor=#82b366;fontSize=11;fontStyle=0;verticalAlign=middle;align=center;" vertex="1" parent="1">
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    <mxCell id="node7" value="机制分析：A3木糖醇形成减少、耐受性提高；木糖异构酶活性4-8倍；启动子-23bp G→A突变" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#d5e8d4;strokeColor=#82b366;fontSize=11;fontStyle=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
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    <mxCell id="node8" value="结论：适应性进化+代谢工程协同改善木糖发酵；木糖醇代谢改变与木糖异构酶上调是关键；进化可提供工程靶点" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#fce5cd;strokeColor=#d79b00;fontSize=12;fontStyle=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
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