<mxfile host="app.diagrams.net"><diagram name="roadmap"><mxGraphModel dx="1200" dy="800" grid="1" gridSize="10" guides="1" tooltips="1" connect="1" arrows="1" fold="1" page="1" pageScale="1" pageWidth="1200" pageHeight="800" math="0" shadow="0" background="#ffffff">
  <root>
    <mxCell id="0" />
    <mxCell id="1" parent="0" />
    <mxCell id="node1" value="研究问题：在酿酒酵母中构建可控且遗传稳定的β-胡萝卜素合成途径" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#dae8fc;strokeColor=#6c8ebf;fontSize=13;fontStyle=1;arcSize=8;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="40" y="40" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node2" value="策略：采用去中心化组装策略，将途径分为多个功能模块整合到不同基因组位点" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#d5e8d4;strokeColor=#82b366;fontSize=13;fontStyle=1;arcSize=8;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="40" y="214" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node3" value="关键改造1：设计pMRI标记可循环整合质粒，支持多次整合和标记回收" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#fff2cc;strokeColor=#d6b656;fontSize=13;fontStyle=1;arcSize=8;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="40" y="388" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node4" value="关键改造2：敲除GAL80，调节途径开关时间" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#fff2cc;strokeColor=#d6b656;fontSize=13;fontStyle=1;arcSize=8;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="64" y="562" width="292" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node5" value="结果：模块化整合效率80-100%，β-胡萝卜素产量7.41 mg/g DCW，较对照提高367倍" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#f8cecc;strokeColor=#b85450;fontSize=13;fontStyle=1;arcSize=8;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="40" y="736" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node6" value="结论：成功构建可控且遗传稳定的β-胡萝卜素合成途径，策略可推广至其他多基因途径" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#ffe6cc;strokeColor=#d79b00;fontSize=13;fontStyle=1;arcSize=8;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="40" y="910" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="edge1" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#6c8ebf;" edge="1" parent="1" source="node1" target="node2">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge2" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#6c8ebf;" edge="1" parent="1" source="node2" target="node3">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge3" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#6c8ebf;" edge="1" parent="1" source="node3" target="node4">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge4" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#6c8ebf;" edge="1" parent="1" source="node4" target="node5">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge5" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#6c8ebf;" edge="1" parent="1" source="node5" target="node6">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
  </root>
</mxGraphModel></diagram></mxfile>