<mxfile host="app.diagrams.net"><diagram name="roadmap"><mxGraphModel dx="1422" dy="794" grid="1" gridSize="10" guides="1" tooltips="1" connect="1" arrows="1" fold="1" page="1" pageScale="1" pageWidth="1400" pageHeight="900" math="0" shadow="0">
  <root>
    <mxCell id="0" />
    <mxCell id="1" parent="0" />

    
    <mxCell id="node1" value="核心问题：β-葡萄糖苷酶活性低，纤维二糖积累抑制水解" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#FF6666;strokeColor=#CC0000;fontColor=#FFFFFF;fontStyle=1;fontSize=13;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="245" y="40" width="340" height="64" as="geometry" />
    </mxCell>

    
    <mxCell id="node2" value="策略：UV+NTG诱变里氏木霉F-522，筛选高产突变株" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#FFCC66;strokeColor=#CC9900;fontColor=#000000;fontStyle=1;fontSize=13;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="245" y="162" width="340" height="64" as="geometry" />
    </mxCell>

    
    <mxCell id="node3" value="关键改造①：七叶灵-铁离子显色平板筛选法" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#99CCFF;strokeColor=#3366CC;fontColor=#000000;fontStyle=1;fontSize=13;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="60" y="284" width="299" height="64" as="geometry" />
    </mxCell>

    
    <mxCell id="node4" value="关键改造②：获得高产突变株V-7（β-葡萄糖苷酶↑6倍）" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#99FF99;strokeColor=#339933;fontColor=#000000;fontStyle=1;fontSize=13;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="245" y="406" width="340" height="64" as="geometry" />
    </mxCell>

    
    <mxCell id="node5" value="结果①：V-7酶学特性——β-G 1.15 U/mL；FPase 0.74 U/mL；CMCase 30.2 U/mL" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#CCCCFF;strokeColor=#6666CC;fontColor=#000000;fontStyle=0;fontSize=12;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="40" y="528" width="340" height="64" as="geometry" />
    </mxCell>

    
    <mxCell id="node6" value="关键改造③：V-7纤维素酶+耐热酵母K. fragilis FT23联用，SSF共发酵" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#99CCFF;strokeColor=#3366CC;fontColor=#000000;fontStyle=1;fontSize=13;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="450" y="528" width="340" height="64" as="geometry" />
    </mxCell>

    
    <mxCell id="node7" value="结果②：SSF产乙醇3.4% (w/v)，较亲本提高36%，时间缩短50%，无纤维二糖残留" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#CCCCFF;strokeColor=#6666CC;fontColor=#000000;fontStyle=0;fontSize=12;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="245" y="650" width="340" height="64" as="geometry" />
    </mxCell>

    
    <mxCell id="node8" value="结果③：水解产物葡萄糖74.0%，纤维二糖仅痕量；乙醇转化率87%理论值" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#CCCCFF;strokeColor=#6666CC;fontColor=#000000;fontStyle=0;fontSize=12;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="245" y="772" width="340" height="64" as="geometry" />
    </mxCell>

    
    <mxCell id="node9" value="结论：高β-G活性是高效转化麦秆为乙醇的关键；V-7+F. fragilis FT23联用显著提升SSF效率" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#66CC66;strokeColor=#006600;fontColor=#FFFFFF;fontStyle=1;fontSize=13;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="430" y="284" width="340" height="64" as="geometry" />
    </mxCell>

    
    <mxCell id="edge1" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#999999;" edge="1" parent="1" source="node1" target="node2">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>

    
    <mxCell id="edge2" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#999999;" edge="1" parent="1" source="node2" target="node3">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>

    
    <mxCell id="edge3" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#999999;" edge="1" parent="1" source="node3" target="node4">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>

    
    <mxCell id="edge4" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#999999;" edge="1" parent="1" source="node4" target="node5">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>

    
    <mxCell id="edge5" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#999999;" edge="1" parent="1" source="node4" target="node6">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>

    
    <mxCell id="edge6" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#999999;" edge="1" parent="1" source="node6" target="node7">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>

    
    <mxCell id="edge7" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#999999;" edge="1" parent="1" source="node7" target="node8">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>

    
    <mxCell id="edge8" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#999999;" edge="1" parent="1" source="node2" target="node9">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>

    
    <mxCell id="edge9" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0;exitY=0.5;exitDx=0;exitDy=0;entryX=0;entryY=0.5;entryDx=0;entryDy=0;dashed=1;dashed=1;dashed=1;strokeColor=#999999;dashed=1;" edge="1" parent="1" source="node5" target="node9">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>

    
    <mxCell id="edge10" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0;exitY=0.5;exitDx=0;exitDy=0;entryX=0;entryY=0.5;entryDx=0;entryDy=0;dashed=1;dashed=1;dashed=1;strokeColor=#999999;dashed=1;" edge="1" parent="1" source="node8" target="node9">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>

  </root>
</mxGraphModel></diagram></mxfile>