<mxfile host="app.diagrams.net"><diagram name="roadmap"><mxGraphModel dx="1400" dy="800" grid="1" gridSize="10" guides="1" tooltips="1" connect="1" arrows="1" fold="1" page="1" pageScale="1" pageWidth="1400" pageHeight="900" math="0" shadow="0">
  <root>
    <mxCell id="0" />
    <mxCell id="1" parent="0" />
    <mxCell id="node1" value="核心问题：三萜环化酶底物混杂性合成双环/三环杂环萜" style="rounded=1;whiteSpace=wrap;html=1;fontSize=14;fillColor=#E8F0FE;strokeColor=#4A90E2;strokeWidth=2;align=center;verticalAlign=middle;fontStyle=1;fontColor=#1A3A6E;" vertex="1" parent="1">
      <mxGeometry x="245" y="40" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node2" value="策略：选用AacSHC和ZmoSHC1两种酶，以功能化短链萜类（C10-C16）为底物" style="rounded=1;whiteSpace=wrap;html=1;fontSize=14;fillColor=#E8F8E0;strokeColor=#7CB342;strokeWidth=2;align=center;verticalAlign=middle;fontStyle=1;fontColor=#2E5E1A;" vertex="1" parent="1">
      <mxGeometry x="40" y="214" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node3" value="关键改造1：底物设计——醇/酮/羧酸末端亲核基团，缩短碳链长度" style="rounded=1;whiteSpace=wrap;html=1;fontSize=13;fillColor=#FFF3E0;strokeColor=#FF9800;strokeWidth=2;align=center;verticalAlign=middle;fontStyle=1;fontColor=#5E3A00;" vertex="1" parent="1">
      <mxGeometry x="450" y="214" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node4" value="关键改造2：酶浓度调控——提高ZmoSHC1浓度促进转化率" style="rounded=1;whiteSpace=wrap;html=1;fontSize=13;fillColor=#FFF3E0;strokeColor=#FF9800;strokeWidth=2;align=center;verticalAlign=middle;fontStyle=1;fontColor=#5E3A00;" vertex="1" parent="1">
      <mxGeometry x="40" y="388" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node5" value="结果1：homofarnesol→ambroxan转化率65.6%，homofarnesic acid→sclareolide转化率22.9%" style="rounded=1;whiteSpace=wrap;html=1;fontSize=13;fillColor=#E0F2F1;strokeColor=#00897B;strokeWidth=2;align=center;verticalAlign=middle;fontStyle=0;fontColor=#003D33;" vertex="1" parent="1">
      <mxGeometry x="450" y="388" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node6" value="结果2：geranylacetone→环醚7a转化率23.0%；nerolidol→(-)-caparrapioxide及8-epi异构体（立体专一性）" style="rounded=1;whiteSpace=wrap;html=1;fontSize=13;fillColor=#E0F2F1;strokeColor=#00897B;strokeWidth=2;align=center;verticalAlign=middle;fontStyle=0;fontColor=#003D33;" vertex="1" parent="1">
      <mxGeometry x="40" y="562" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node7" value="创新点：首次证明SHC催化生成内酯/环烯醇醚；无需焦磷酸激活（class II机制）" style="rounded=1;whiteSpace=wrap;html=1;fontSize=13;fillColor=#FDEBEC;strokeColor=#E53935;strokeWidth=2;align=center;verticalAlign=middle;fontStyle=1;fontColor=#6E1010;" vertex="1" parent="1">
      <mxGeometry x="450" y="562" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node8" value="结论：三萜环化酶为通用Brønsted酸催化剂，底物工程策略合成杂环天然产物，适用于香料绿色合成" style="rounded=1;whiteSpace=wrap;html=1;fontSize=14;fillColor=#E3F2FD;strokeColor=#1E88E5;strokeWidth=2;align=center;verticalAlign=middle;fontStyle=1;fontColor=#0D4F8B;" vertex="1" parent="1">
      <mxGeometry x="245" y="736" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="edge1" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#4A90E2;" edge="1" parent="1" source="node1" target="node2">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge2" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#4A90E2;" edge="1" parent="1" source="node1" target="node3">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge3" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#4A90E2;" edge="1" parent="1" source="node2" target="node4">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge4" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#00897B;" edge="1" parent="1" source="node3" target="node5">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge5" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#00897B;" edge="1" parent="1" source="node4" target="node6">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge6" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#E53935;" edge="1" parent="1" source="node5" target="node7">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge7" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=1;exitY=0.5;exitDx=0;exitDy=0;entryX=0;entryY=0.5;entryDx=0;entryDy=0;strokeColor=#E53935;" edge="1" parent="1" source="node6" target="node7">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge8" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#1E88E5;" edge="1" parent="1" source="node7" target="node8">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
  </root>
</mxGraphModel></diagram></mxfile>