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    <mxCell id="node2" value="策略：敲除硫胺素合成调控基因（THI2 / THI3），降低PDC活性，优化硫胺素与碳氮比，将碳流由乙醇转向丙酮酸" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#d5e8d4;strokeColor=#82b366;fontSize=12;fontStyle=1;verticalAlign=middle;" vertex="1" parent="1">
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    <mxCell id="node3" value="关键改造①：敲除THI2正调控因子，降低THI基因表达，减少ThDP供给，抑制PDC活性" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#ffe6cc;strokeColor=#d79b00;fontSize=12;fontStyle=1;verticalAlign=middle;" vertex="1" parent="1">
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    <mxCell id="node4" value="关键改造②：敲除THI3全局调控因子，影响所有硫胺素调控基因表达" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#ffe6cc;strokeColor=#d79b00;fontSize=12;fontStyle=1;verticalAlign=middle;" vertex="1" parent="1">
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    <mxCell id="node6" value="结果①：THI2/THI3敲除后丙酮酸产量提高3.12倍/2.18倍，乙醇降低89.9%/81.0%" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#dae8fc;strokeColor=#6c8ebf;fontSize=12;fontStyle=1;verticalAlign=middle;" vertex="1" parent="1">
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    <mxCell id="node7" value="结果②：最优C:N=35:2条件下，96 h丙酮酸达8.21 g/L（提高9.0倍），乙醇降低58.06%" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#dae8fc;strokeColor=#6c8ebf;fontSize=12;fontStyle=1;verticalAlign=middle;" vertex="1" parent="1">
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    <mxCell id="node8" value="结果③：THI2缺失48 h PDC比活力下降67.0%，0.04 μM硫胺素下PDC活性回升10.5%，碳流约16.2%由乙醇转向丙酮酸" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#dae8fc;strokeColor=#6c8ebf;fontSize=12;fontStyle=1;verticalAlign=middle;" vertex="1" parent="1">
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    <mxCell id="node9" value="结论：调控硫胺素合成可有效提高酿酒酵母丙酮酸产量并降低乙醇副产物，为羧酸类产品提供前体丙酮酸，替代PDC直接敲除的新策略" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#e1d5e7;strokeColor=#9673a6;fontSize=12;fontStyle=1;verticalAlign=middle;" vertex="1" parent="1">
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