<mxfile host="app.diagrams.net"><diagram name="roadmap"><mxGraphModel dx="1422" dy="794" grid="1" gridSize="10" guides="1" tooltips="1" connect="1" arrows="1" fold="1" page="1" pageScale="1" pageWidth="1600" pageHeight="900" math="0" shadow="0">
  <root>
    <mxCell id="0" />
    <mxCell id="1" parent="0" />
    <mxCell id="node1" value="研究问题：烟草叶绿体CMO途径合成甘氨酸甜菜碱的通量限制因素？" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#dae8fc;strokeColor=#6c8ebf;fontSize=13;fontStyle=1;fontColor=#1a1a1a;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="40" y="40" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node2" value="策略：构建不同活性的叶绿体CMO转基因烟草；另建胞质COX途径作对照" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#d5e8d4;strokeColor=#82b366;fontSize=13;fontStyle=1;fontColor=#1a1a1a;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="40" y="214" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node3" value="关键改造：优化CMO表达元件获得高活性（8 pkat/mg蛋白）；胞质表达COX+BADH" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#fff2cc;strokeColor=#d6b656;fontSize=13;fontStyle=1;fontColor=#1a1a1a;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="40" y="388" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node4" value="结果：CMO活性提高100倍但甜菜碱积累无增加；COX途径积累量高10倍" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#f8cecc;strokeColor=#b85450;fontSize=13;fontStyle=1;fontColor=#1a1a1a;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="40" y="562" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node5" value="机制分析：[14C]胆碱标记+动力学建模揭示叶绿体胆碱跨膜转运为非饱和低通量瓶颈" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#e1d5e7;strokeColor=#9673a6;fontSize=13;fontStyle=1;fontColor=#1a1a1a;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="40" y="736" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node6" value="结论：叶绿体胆碱输入是主要瓶颈；短期用胞质COX途径，长期建议表达高亲和力胆碱转运蛋白（如E. coli BetT）" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#d5e8d4;strokeColor=#82b366;fontSize=13;fontStyle=1;fontColor=#1a1a1a;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="40" y="910" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="edge1" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#6c8ebf;" edge="1" source="node1" target="node2" parent="1">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge2" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#82b366;" edge="1" source="node2" target="node3" parent="1">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge3" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#d6b656;" edge="1" source="node3" target="node4" parent="1">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge4" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#b85450;dashed=1;" edge="1" source="node4" target="node5" parent="1">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge5" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#9673a6;" edge="1" source="node5" target="node6" parent="1">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
  </root>
</mxGraphModel></diagram></mxfile>