<mxfile host="app.diagrams.net"><diagram name="roadmap"><mxGraphModel dx="1200" dy="800" grid="1" gridSize="10" guides="1" tooltips="1" connect="1" arrows="1" fold="1" page="1" pageScale="1" pageWidth="1000" pageHeight="700" math="0" shadow="0">
<root>
<mxCell id="0" />
<mxCell id="1" parent="0" />

<mxCell id="n1" value="研究问题：链霉菌原生质体融合后基因重组的基本特征？" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#DAE8FC;strokeColor=#6C8EBF;fontSize=12;fontStyle=1verticalAlign=middle;align=center;" vertex="1" parent="1">
<mxGeometry x="381" y="40" width="340" height="64" as="geometry" />
</mxCell>
<mxCell id="n2" value="策略：PEG诱导原生质体融合+六因子杂交分析" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#D5E8D4;strokeColor=#82B366;fontSize=12;fontStyle=1verticalAlign=middle;align=center;" vertex="1" parent="1">
<mxGeometry x="224" y="162" width="306" height="64" as="geometry" />
</mxCell>
<mxCell id="n3" value="关键改造：DNase处理排除转化干扰" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#FFF2CC;strokeColor=#D6B656;fontSize=12;fontStyle=1verticalAlign=middle;align=center;" vertex="1" parent="1">
<mxGeometry x="40" y="284" width="243" height="64" as="geometry" />
</mxCell>
<mxCell id="n4" value="六因子杂交：重组频率17%（平均14.4%）" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#F8CECC;strokeColor=#B85450;fontSize=12;fontStyle=0verticalAlign=middle;align=center;" vertex="1" parent="1">
<mxGeometry x="600" y="162" width="278" height="64" as="geometry" />
</mxCell>
<mxCell id="n5" value="交换模式：双交换76.25%、四交换23.25%、六交换0.5%" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#F8CECC;strokeColor=#B85450;fontSize=12;fontStyle=0verticalAlign=middle;align=center;" vertex="1" parent="1">
<mxGeometry x="353" y="284" width="340" height="64" as="geometry" />
</mxCell>
<mxCell id="n6" value="四亲本融合：三亲本重组子4.0×10⁻⁴、四亲本1.2×10⁻⁷" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#F8CECC;strokeColor=#B85450;fontSize=12;fontStyle=0verticalAlign=middle;align=center;" vertex="1" parent="1">
<mxGeometry x="193" y="406" width="340" height="64" as="geometry" />
</mxCell>
<mxCell id="n7" value="单菌落分析：12%含重组子，无同时含两亲本" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#F8CECC;strokeColor=#B85450;fontSize=12;fontStyle=0verticalAlign=middle;align=center;" vertex="1" parent="1">
<mxGeometry x="763" y="284" width="299" height="64" as="geometry" />
</mxCell>
<mxCell id="n8" value="结论：高频率多轮多亲本重组，工具价值显著" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#E1D5E7;strokeColor=#9673A6;fontSize=12;fontStyle=1verticalAlign=middle;align=center;" vertex="1" parent="1">
<mxGeometry x="603" y="406" width="306" height="64" as="geometry" />
</mxCell>

<mxCell id="e1" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#6C8EBF;" edge="1" source="n1" target="n2" parent="1">
<mxGeometry relative="1" as="geometry" />
</mxCell>
<mxCell id="e2" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#6C8EBF;" edge="1" source="n2" target="n3" parent="1">
<mxGeometry relative="1" as="geometry" />
</mxCell>
<mxCell id="e3" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#6C8EBF;" edge="1" source="n1" target="n4" parent="1">
<mxGeometry relative="1" as="geometry" />
</mxCell>
<mxCell id="e4" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#6C8EBF;" edge="1" source="n2" target="n5" parent="1">
<mxGeometry relative="1" as="geometry" />
</mxCell>
<mxCell id="e5" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#6C8EBF;" edge="1" source="n3" target="n6" parent="1">
<mxGeometry relative="1" as="geometry" />
</mxCell>
<mxCell id="e6" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#6C8EBF;" edge="1" source="n4" target="n7" parent="1">
<mxGeometry relative="1" as="geometry" />
</mxCell>
<mxCell id="e7" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#6C8EBF;" edge="1" source="n5" target="n8" parent="1">
<mxGeometry relative="1" as="geometry" />
</mxCell>
<mxCell id="e8" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=1;exitY=0.5;exitDx=0;exitDy=0;entryX=0;entryY=0.5;entryDx=0;entryDy=0;strokeColor=#6C8EBF;" edge="1" source="n6" target="n8" parent="1">
<mxGeometry relative="1" as="geometry" />
</mxCell>
<mxCell id="e9" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#6C8EBF;" edge="1" source="n7" target="n8" parent="1">
<mxGeometry relative="1" as="geometry" />
</mxCell>
</root>
</mxGraphModel></diagram></mxfile>