<mxfile host="app.diagrams.net"><diagram name="roadmap"><mxGraphModel dx="1200" dy="800" grid="1" gridSize="10" guides="1" tooltips="1" connect="1" arrows="1" fold="1" page="1" pageScale="1" pageWidth="1400" pageHeight="900" math="0" shadow="0">
  <root>
    <mxCell id="0" />
    <mxCell id="1" parent="0" />
    <mxCell id="node1" value="研究问题：开发高效广宿主转座子突变系统" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#FFCCCC;strokeColor=#CC0000;fontSize=14;fontStyle=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="64" y="40" width="292" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node2" value="策略：构建pRL27载体（高活性Tn5转座酶+RP4启动子）" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#FFFFCC;strokeColor=#CC9900;fontSize=13;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="40" y="162" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node3" value="接合转移导入宿主菌，随机插入突变" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#CCE5FF;strokeColor=#0066CC;fontSize=13;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="85" y="284" width="250" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node4" value="筛选：约17,000个突变体，44个色素缺陷突变" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#D9FFCC;strokeColor=#339900;fontSize=13;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="57" y="406" width="306" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node5" value="关键改造：oriR6K一步克隆插入位点+Km抗性标记" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#E5CCFF;strokeColor=#660099;fontSize=13;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="46" y="528" width="327" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node6" value="结果：鉴定crtYIB操纵子、crtX及ispA等基因" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#CCFFFF;strokeColor=#009999;fontSize=13;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="57" y="650" width="306" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node7" value="发现：9-bp靶位点重复，转座随机性验证" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#FFE5CC;strokeColor=#FF6600;fontSize=13;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="71" y="772" width="278" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node8" value="结论：广宿主Tn5系统高效可靠，阐明zeaxanthin dirhamnoside合成基因" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#FFCCE5;strokeColor=#CC0066;fontSize=14;fontStyle=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="40" y="894" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="edge1" edge="1" parent="1" source="node1" target="node2" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#333333;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge2" edge="1" parent="1" source="node2" target="node3" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#333333;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge3" edge="1" parent="1" source="node3" target="node4" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#333333;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge4" edge="1" parent="1" source="node4" target="node5" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#333333;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge5" edge="1" parent="1" source="node5" target="node6" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#333333;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge6" edge="1" parent="1" source="node6" target="node7" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#333333;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge7" edge="1" parent="1" source="node7" target="node8" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#333333;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
  </root>
</mxGraphModel></diagram></mxfile>