<mxfile host="app.diagrams.net"><diagram name="roadmap"><mxGraphModel dx="1422" dy="794" grid="1" gridSize="10" guides="1" tooltips="1" connect="1" arrows="1" fold="1" page="1" pageScale="1" pageWidth="1400" pageHeight="900" math="0" shadow="0">
  <root>
    <mxCell id="0" />
    <mxCell id="1" parent="0" />
    
    
    <mxCell id="n1" value="研究问题：细胞膜通透性限制、酶失活与相转移限制制约α-蒎烯氧化物转化" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#DAE8FC;strokeColor=#6C8EBF;fontSize=14;fontStyle=1;verticalAlign=middle;align=center;arcSize=8;" vertex="1" parent="1">
      <mxGeometry x="655" y="40" width="340" height="64" as="geometry" />
    </mxCell>
    
    <mxCell id="n2" value="策略：冷冻和有机溶剂透化细胞释放酶并改善物质跨膜运输" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#D5E8D4;strokeColor=#82B366;fontSize=14;fontStyle=1;verticalAlign=middle;align=center;arcSize=8;" vertex="1" parent="1">
      <mxGeometry x="655" y="162" width="340" height="64" as="geometry" />
    </mxCell>
    
    <mxCell id="n3" value="关键改造：氯仿/甲苯/乙醚透化方式系统评估与筛选" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#FFF2CC;strokeColor=#D6B656;fontSize=14;fontStyle=0;verticalAlign=middle;align=center;arcSize=8;" vertex="1" parent="1">
      <mxGeometry x="655" y="284" width="340" height="64" as="geometry" />
    </mxCell>
    
    <mxCell id="n4" value="关键发现①：乙醚透化→初始速率1280 μmol/min/g（最优）" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#F8CECC;strokeColor=#B85450;fontSize=14;fontStyle=0;verticalAlign=middle;align=center;arcSize=8;" vertex="1" parent="1">
      <mxGeometry x="40" y="406" width="340" height="64" as="geometry" />
    </mxCell>
    
    <mxCell id="n5" value="关键发现②：酶失活由产物（isonovalal/副产物）引起，不可逆且浓度依赖" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#F8CECC;strokeColor=#B85450;fontSize=14;fontStyle=0;verticalAlign=middle;align=center;arcSize=8;" vertex="1" parent="1">
      <mxGeometry x="450" y="406" width="340" height="64" as="geometry" />
    </mxCell>
    
    <mxCell id="n6" value="关键发现③：双相体系自发形成稳定水包油乳液→无相转移限制" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#F8CECC;strokeColor=#B85450;fontSize=14;fontStyle=0;verticalAlign=middle;align=center;arcSize=8;" vertex="1" parent="1">
      <mxGeometry x="860" y="406" width="340" height="64" as="geometry" />
    </mxCell>
    
    <mxCell id="n7" value="关键改造：底物+细胞双补料策略克服酶不可逆失活" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#FFF2CC;strokeColor=#D6B656;fontSize=14;fontStyle=0;verticalAlign=middle;align=center;arcSize=8;" vertex="1" parent="1">
      <mxGeometry x="1270" y="406" width="340" height="64" as="geometry" />
    </mxCell>
    
    <mxCell id="n8" value="结果：2.5h产2.63M；20h双补料产3.29M；常数转化率108mmol/g" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#E1D5E7;strokeColor=#9673A6;fontSize=14;fontStyle=1;verticalAlign=middle;align=center;arcSize=8;" vertex="1" parent="1">
      <mxGeometry x="655" y="528" width="340" height="64" as="geometry" />
    </mxCell>
    
    <mxCell id="n9" value="结论：透化+补料策略可实现高浓度产物，系统可放大，具备工业应用潜力" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#FAD9D5;strokeColor=#AE4132;fontSize=14;fontStyle=1;verticalAlign=middle;align=center;arcSize=8;" vertex="1" parent="1">
      <mxGeometry x="655" y="650" width="340" height="64" as="geometry" />
    </mxCell>
    
    
    <mxCell id="e1" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#4D4D4D;" edge="1" parent="1" source="n1" target="n2">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    
    <mxCell id="e2" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#4D4D4D;" edge="1" parent="1" source="n2" target="n3">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    
    <mxCell id="e3" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#4D4D4D;" edge="1" parent="1" source="n3" target="n4">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    
    <mxCell id="e4" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#4D4D4D;dashed=1;" edge="1" parent="1" source="n3" target="n5">
      <mxGeometry relative="1" as="geometry">
        </mxGeometry>
    </mxCell>
    
    <mxCell id="e5" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#4D4D4D;dashed=1;" edge="1" parent="1" source="n3" target="n6">
      <mxGeometry relative="1" as="geometry">
        </mxGeometry>
    </mxCell>
    
    <mxCell id="e6" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#4D4D4D;dashed=1;" edge="1" parent="1" source="n3" target="n7">
      <mxGeometry relative="1" as="geometry">
        </mxGeometry>
    </mxCell>
    
    <mxCell id="e7" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#4D4D4D;" edge="1" parent="1" source="n5" target="n8">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    
    <mxCell id="e8" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#4D4D4D;" edge="1" parent="1" source="n6" target="n8">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    
    <mxCell id="e9" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#4D4D4D;" edge="1" parent="1" source="n7" target="n8">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    
    <mxCell id="e10" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#4D4D4D;" edge="1" parent="1" source="n8" target="n9">
      <mxGeometry relative="1" as="geometry">
        </mxGeometry>
    </mxCell>
    
  </root>
</mxGraphModel></diagram></mxfile>