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    <mxCell id="n1" value="研究问题：需氧MR-1还原CT的分子机制？甲萘醌/DHNA是否参与？" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#D6EAF8;strokeColor=#2980B9;fontSize=13;fontColor=#1A1A2E;fontStyle=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
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    <mxCell id="n2" value="策略：构建menF/menB/menA/menG突变体，对比需氧/厌氧CT转化活性" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#D5F5E3;strokeColor=#27AE60;fontSize=13;fontColor=#1A1A2E;fontStyle=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
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    <mxCell id="n3" value="关键改造：基因插入失活menF/menB/menA/menG；DHNA回补实验；体外化学验证" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#FDEBD0;strokeColor=#E67E22;fontSize=13;fontColor=#1A1A2E;fontStyle=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
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    <mxCell id="n4" value="结果1：需氧CT转化k'（野生型0.219 vs menF 0.011, menB 0.009, menA 0.216, menG 0.187）" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#E8DAEF;strokeColor=#8E44AD;fontSize=12;fontColor=#1A1A2E;verticalAlign=middle;align=center;" vertex="1" parent="1">
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    <mxCell id="n5" value="结果2：DHNA回补menF/menB突变体恢复CT转化活性（k'=0.104/0.126）" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#D6EAF8;strokeColor=#2980B9;fontSize=12;fontColor=#1A1A2E;verticalAlign=middle;align=center;" vertex="1" parent="1">
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    <mxCell id="n6" value="结果3：厌氧条件下各men突变体k'≈野生型（0.087–0.093 vs 0.110），另生脱氯因子" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#D5F5E3;strokeColor=#27AE60;fontSize=12;fontColor=#1A1A2E;verticalAlign=middle;align=center;" vertex="1" parent="1">
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    <mxCell id="n7" value="结果4：DHNA体外（0.15–15 μM）不还原CT；需氧menA/menG突变体产13–26%氯仿" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#FDEBD0;strokeColor=#E67E22;fontSize=12;fontColor=#1A1A2E;verticalAlign=middle;align=center;" vertex="1" parent="1">
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    <mxCell id="n8" value="结论：需氧活性分子为DHNA衍生物（非甲萘醌）；厌氧为另一无关分子；均生成氯仿" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#FADBD8;strokeColor=#C0392B;fontSize=13;fontColor=#1A1A2E;fontStyle=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
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