<mxfile host="app.diagrams.net"><diagram name="roadmap"><mxGraphModel dx="1422" dy="762" grid="1" gridSize="10" guides="1" tooltips="1" connect="1" arrows="1" fold="1" page="1" pageScale="1" pageWidth="1169" pageHeight="826" math="0" shadow="0">
  <root>
    <mxCell id="0" />
    <mxCell id="1" parent="0" />
    <mxCell id="node1" value="核心问题：异丁醇产量受毒性限制" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#dae8fc;strokeColor=#6c8ebf;fontSize=13;fontStyle=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="256" y="40" width="236" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node2" value="策略：1L反应器+气提原位产物移除，比较三菌株&#10;结果3：30°C优于37°C，AdhA活性受限" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#d5e8d4;strokeColor=#82b366;fontSize=13;fontStyle=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="210" y="162" width="327" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node3" value="关键改造：工程菌JCL260（敲除6个基因+过表达5个酶）" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#fff2cc;strokeColor=#d6b656;fontSize=12;fontStyle=0;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="204" y="284" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node4" value="结果1：JCL260在30°C产50.8 g/L异丁醇" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#f8cecc;strokeColor=#b85450;fontSize=13;fontStyle=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="40" y="406" width="278" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node5" value="结果2：耐受菌SA481产率最低（23 g/L）" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#f8cecc;strokeColor=#b85450;fontSize=12;fontStyle=0;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="235" y="528" width="278" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node6" value="结果3：30°C优于37°C，AdhA活性是关键" parent="1" /> 
    <mxCell id="node7" value="结论：气提+30°C实现高效生产，耐受菌无优势" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#e1d5e7;strokeColor=#9673a6;fontSize=13;fontStyle=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="388" y="406" width="320" height="64" as="geometry" />
    </mxCell>
    <mxCell id="edge1" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#6c8ebf;" edge="1" parent="1" source="node1" target="node2">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge2" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#6c8ebf;" edge="1" parent="1" source="node2" target="node3">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge3" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#b85450;" edge="1" parent="1" source="node3" target="node4">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge4" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#b85450;" edge="1" parent="1" source="node4" target="node5">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge5" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;strokeColor=#b85450;strokeWidth=2;exitX=0.5;exitY=1;entryX=0.5;entryY=0;" edge="1" parent="1" source="node5" target="node6">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge6" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;strokeColor=#9673a6;strokeWidth=2;exitX=1;exitY=0.5;entryX=0;entryY=0.5;exitDx=20;exitDy=0;entryDx=-20;entryDy=0;" edge="1" parent="1" source="node6" target="node7">
      <mxGeometry relative="1" as="geometry">
        <Array as="points">
          <mxPoint x="690" y="375" />
          <mxPoint x="690" y="235" />
          <mxPoint x="800" y="235" />
        </Array>
      </mxGeometry>
    </mxCell>
    <mxCell id="edge7" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#9673a6;dashed=1;" edge="1" parent="1" source="node3" target="node7">
      <mxGeometry relative="1" as="geometry">
        </mxGeometry>
    </mxCell>
  </root>
</mxGraphModel></diagram></mxfile>