<mxfile host="app.diagrams.net"><diagram name="roadmap"><mxGraphModel dx="1200" dy="800" grid="1" gridSize="10" guides="1" tooltips="1" connect="1" arrows="1" fold="1" page="1" pageScale="1" pageWidth="1200" pageHeight="800" math="0" shadow="0">
  <root>
    <mxCell id="0" />
    <mxCell id="1" parent="0" />
    
    
    <mxCell id="node1" value="副产物乳酸竞争丙酮酸和NADH，降低2,3-丁二醇产量" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#dae8fc;strokeColor=#6c8ebf;fontSize=12;fontStyle=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="40" y="40" width="340" height="64" as="geometry" />
    </mxCell>
    
    
    <mxCell id="node2" value="λRed重组法敲除ldhA基因" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#d5e8d4;strokeColor=#82b366;fontSize=12;fontStyle=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="805" y="162" width="210" height="64" as="geometry" />
    </mxCell>
    
    
    <mxCell id="node3" value="产气肠杆菌KCTC2190 ΔldhA工程菌株构建" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#fff2cc;strokeColor=#d6b656;fontSize=12;fontStyle=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="768" y="284" width="285" height="64" as="geometry" />
    </mxCell>
    
    
    <mxCell id="node4" value="分批发酵验证：乳酸降97%，2,3-丁二醇产量提升16.7%" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#ffe6cc;strokeColor=#d79b00;fontSize=12;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="740" y="406" width="340" height="64" as="geometry" />
    </mxCell>
    
    
    <mxCell id="node5" value="补料发酵：产量达69.12 g/L，提高28.5%" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#ffe6cc;strokeColor=#d79b00;fontSize=12;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="631" y="528" width="278" height="64" as="geometry" />
    </mxCell>
    
    
    <mxCell id="node6" value="添加酪蛋白氨基酸＋提高通气量优化培养" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#fff2cc;strokeColor=#d6b656;fontSize=12;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="597" y="650" width="278" height="64" as="geometry" />
    </mxCell>
    
    
    <mxCell id="node7" value="最优产量118.05 g/L，生产力3.2 g/L/h" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#f8cecc;strokeColor=#b85450;fontSize=12;fontStyle=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="774" y="772" width="271" height="64" as="geometry" />
    </mxCell>
    
    
    <mxCell id="node8" value="六种碳源消耗和生物量均提升" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#ffe6cc;strokeColor=#d79b00;fontSize=12;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="979" y="528" width="210" height="64" as="geometry" />
    </mxCell>
    
    
    <mxCell id="node9" value="结论：代谢工程有效提高2,3-丁二醇生产" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#dae8fc;strokeColor=#6c8ebf;fontSize=13;fontStyle=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="945" y="650" width="278" height="64" as="geometry" />
    </mxCell>
    
    
    <mxCell id="edge1" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#6c8ebf;" edge="1" parent="1" source="node1" target="node2">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    
    
    <mxCell id="edge2" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#82b366;" edge="1" parent="1" source="node2" target="node3">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    
    
    <mxCell id="edge3" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#d6b656;" edge="1" parent="1" source="node3" target="node4">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    
    
    <mxCell id="edge4" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#d79b00;" edge="1" parent="1" source="node4" target="node5">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    
    
    <mxCell id="edge5" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#d6b656;" edge="1" parent="1" source="node5" target="node6">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    
    
    <mxCell id="edge6" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#b85450;" edge="1" parent="1" source="node6" target="node7">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    
    
    <mxCell id="edge7" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#d79b00;dashed=1;" edge="1" parent="1" source="node4" target="node8">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    
    
    <mxCell id="edge8" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#6c8ebf;dashed=1;" edge="1" parent="1" source="node8" target="node9">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    
    
    <mxCell id="edge9" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0;exitY=0.5;exitDx=0;exitDy=0;entryX=0;entryY=0.5;entryDx=0;entryDy=0;dashed=1;dashed=1;dashed=1;strokeColor=#6c8ebf;dashed=1;" edge="1" parent="1" source="node7" target="node9">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    
    
    <mxCell id="label1" value="问题" style="text;html=1;fontSize=11;align=center;fillColor=none;fontColor=#6c8ebf;fontStyle=1;whiteSpace=wrap;html=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="450" y="40" width="210" height="64" as="geometry" />
    </mxCell>
    <mxCell id="label2" value="策略" style="text;html=1;fontSize=11;align=center;fillColor=none;fontColor=#82b366;fontStyle=1;whiteSpace=wrap;html=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="730" y="40" width="210" height="64" as="geometry" />
    </mxCell>
    <mxCell id="label3" value="改造" style="text;html=1;fontSize=11;align=center;fillColor=none;fontColor=#d6b656;fontStyle=1;whiteSpace=wrap;html=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="1010" y="40" width="210" height="64" as="geometry" />
    </mxCell>
    <mxCell id="label4" value="结果" style="text;html=1;fontSize=11;align=center;fillColor=none;fontColor=#d79b00;fontStyle=1;whiteSpace=wrap;html=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="1290" y="40" width="210" height="64" as="geometry" />
    </mxCell>
    <mxCell id="label5" value="结论" style="text;html=1;fontSize=11;align=center;fillColor=none;fontColor=#6c8ebf;fontStyle=1;whiteSpace=wrap;html=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="1570" y="40" width="210" height="64" as="geometry" />
    </mxCell>
    
  </root>
</mxGraphModel></diagram></mxfile>