<mxfile host="app.diagrams.net"><diagram name="roadmap"><mxGraphModel dx="1200" dy="800" grid="1" gridSize="10" guides="1" tooltips="1" connect="1" arrows="1" fold="1" page="1" pageScale="1" pageWidth="1200" pageHeight="800" math="0" shadow="0">
  <root>
    <mxCell id="0" />
    <mxCell id="1" parent="0" />
    <mxCell id="node1" value="核心问题：L-泛酰内酯立体选择性氧化及D-泛酰内酯立体转化" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#FFE6CC;strokeColor=#E69138;fontSize=12;fontStyle=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="245" y="40" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node2" value="策略：从红球菌克隆lpldh基因，构建过表达载体并导入原始菌株" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#D9E1F2;strokeColor=#6D9EEB;fontSize=12;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="245" y="162" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node3" value="关键改造1：lpldh基因过表达载体pKPLDH构建与导入" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#D9EAD3;strokeColor=#6AA84F;fontSize=12;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="245" y="284" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node4" value="关键改造2：基因敲除验证lpldh功能" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#D9EAD3;strokeColor=#6AA84F;fontSize=12;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="85" y="406" width="250" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node5" value="关键改造3：偶联E. coli（KPA还原酶+葡萄糖脱氢酶）" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#D9EAD3;strokeColor=#6AA84F;fontSize=12;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="40" y="528" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node6" value="结果：LPLDH活性提高约6倍，1.15 M L-PL转化率达80.3%" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#F4CCCC;strokeColor=#CC0000;fontSize=12;fontStyle=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="405" y="406" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node7" value="结果：0.768 M L-PL经偶联立体转化得0.707 M D-PL，产率92%" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#F4CCCC;strokeColor=#CC0000;fontSize=12;fontStyle=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="245" y="650" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node8" value="结论：实现L-PL→KPL→KPA→D-PA三步偶联立体转化，具工业潜力" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#FFF2CC;strokeColor=#BF9000;fontSize=12;fontStyle=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="450" y="528" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="edge1" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#666666;" edge="1" parent="1" source="node1" target="node2">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge2" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#666666;" edge="1" parent="1" source="node2" target="node3">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge3" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#666666;dashed=1;" edge="1" parent="1" source="node3" target="node4">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge4" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#666666;dashed=1;" edge="1" parent="1" source="node4" target="node5">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge5" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#666666;" edge="1" parent="1" source="node3" target="node6">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge6" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#666666;" edge="1" parent="1" source="node5" target="node7">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge7" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#666666;" edge="1" parent="1" source="node6" target="node8">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge8" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0;exitY=0.5;exitDx=0;exitDy=0;entryX=0;entryY=0.5;entryDx=0;entryDy=0;dashed=1;dashed=1;dashed=1;strokeColor=#666666;" edge="1" parent="1" source="node7" target="node8">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
  </root>
</mxGraphModel></diagram></mxfile>