<mxfile host="app.diagrams.net"><diagram name="roadmap"><mxGraphModel dx="1422" dy="762" grid="1" gridSize="10" guides="1" tooltips="1" connect="1" arrows="1" fold="1" page="1" pageScale="1" pageWidth="1600" pageHeight="900" math="0" shadow="0">
  <root>
    <mxCell id="0" />
    <mxCell id="1" parent="0" />
    <mxCell id="n1" value="研究问题：以甲醇为唯一碳源，通过M. extorquens AM1代谢工程合成PHA共聚物（非均聚P(3HB)）" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#DAE8FC;strokeColor=#6C8EBF;fontSize=12;fontStyle=1;verticalAlign=middle;arcSize=10;" vertex="1" parent="1">
      <mxGeometry x="40" y="40" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="e1" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#6C8EBF;" edge="1" parent="1" source="n1" target="n2">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="n2" value="策略1：利用钴缺乏条件激活EMC途径积累丙酰-CoA" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#F8CECC;strokeColor=#B85450;fontSize=11;verticalAlign=middle;arcSize=12;" vertex="1" parent="1">
      <mxGeometry x="43" y="162" width="334" height="64" as="geometry" />
    </mxCell>
    <mxCell id="e2" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#6C8EBF;" edge="1" parent="1" source="n2" target="n3">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="n3" value="策略2：替换phaC为phaC_Ac（广底物特异性）" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#F8CECC;strokeColor=#B85450;fontSize=11;verticalAlign=middle;arcSize=12;" vertex="1" parent="1">
      <mxGeometry x="57" y="284" width="306" height="64" as="geometry" />
    </mxCell>
    <mxCell id="e3" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#6C8EBF;" edge="1" parent="1" source="n3" target="n4">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="n4" value="关键改造：敲除pccA增强3HV比例；过表达phaAB" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#FFF2CC;strokeColor=#D6B656;fontSize=11;verticalAlign=middle;arcSize=12;" vertex="1" parent="1">
      <mxGeometry x="50" y="406" width="320" height="64" as="geometry" />
    </mxCell>
    <mxCell id="e4" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#6C8EBF;" edge="1" parent="1" source="n4" target="n5">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="n5" value="关键改造：敲除depA/depB/depC阻断PHA降解" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#FFF2CC;strokeColor=#D6B656;fontSize=11;verticalAlign=middle;arcSize=12;" vertex="1" parent="1">
      <mxGeometry x="60" y="528" width="299" height="64" as="geometry" />
    </mxCell>
    <mxCell id="e5" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#D6B656;" edge="1" parent="1" source="n5" target="n6">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="n6" value="结果1：野生型合成P(3HB-co-3HV)（1-6 mol% 3HV）" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#D5E8D4;strokeColor=#82B366;fontSize=11;verticalAlign=middle;arcSize=12;" vertex="1" parent="1">
      <mxGeometry x="40" y="650" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="e6" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#82B366;" edge="1" parent="1" source="n6" target="n7">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="n7" value="结果2：phaC_Ac菌株PHA含量32.6 wt%（野生型4倍）且含3HHx" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#D5E8D4;strokeColor=#82B366;fontSize=11;verticalAlign=middle;arcSize=12;" vertex="1" parent="1">
      <mxGeometry x="40" y="772" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="e7" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#82B366;" edge="1" parent="1" source="n7" target="n8">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="n8" value="结果3：pccA敲除使3HV比例达77.7 mol%；phaAB过表达使含量达43.6 wt%" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#D5E8D4;strokeColor=#82B366;fontSize=11;verticalAlign=middle;arcSize=12;" vertex="1" parent="1">
      <mxGeometry x="40" y="894" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="e8" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#82B366;" edge="1" parent="1" source="n8" target="n9">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="n9" value="结论：EMC途径是PHA共聚物前体平台，甲醇可作碳源生产共聚物" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#E1D5E7;strokeColor=#9673A6;fontSize=12;fontStyle=1;verticalAlign=middle;arcSize=10;" vertex="1" parent="1">
      <mxGeometry x="40" y="1016" width="340" height="64" as="geometry" />
    </mxCell>
  </root>
</mxGraphModel></diagram></mxfile>