<mxfile host="app.diagrams.net"><diagram name="roadmap"><mxGraphModel>
  <root>
    <mxCell id="0" />
    <mxCell id="1" parent="0" />
    <mxCell id="core_problem" value="&lt;b&gt;科学问题&lt;/b&gt;：肺炎克雷伯氏菌2,3-丁二醇立体异构体形成机制" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#dae8fc;strokeColor=#6c8ebf;fontSize=12;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="450" y="40" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="strategy" value="&lt;b&gt;研究策略&lt;/b&gt;：基因敲除+异源表达+体外酶学+产物分析" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#d5e8d4;strokeColor=#82b366;fontSize=12;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="450" y="162" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="edge1" edge="1" parent="1" source="core_problem" target="strategy" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="budA" value="&lt;b&gt;ΔbudA突变&lt;/b&gt;（α-乙酰乳酸脱羧酶）：R-乙偶姻合成受阻，2R,3R-BDO减少" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#fff2cc;strokeColor=#d6b656;fontSize=12;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="40" y="284" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="edge2" edge="1" parent="1" source="strategy" target="budA" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="budC" value="&lt;b&gt;ΔbudC突变&lt;/b&gt;（丁二醇脱氢酶）：丧失2S,3S-BDO，R-乙偶姻累积，2R,3R-BDO保留" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#fff2cc;strokeColor=#d6b656;fontSize=12;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="450" y="284" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="edge3" edge="1" parent="1" source="strategy" target="budC" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="dhaD" value="&lt;b&gt;ΔdhaD突变&lt;/b&gt;（甘油脱氢酶）：完全丧失2R,3R-BDO合成★关键酶" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#fff2cc;strokeColor=#d6b656;fontSize=12;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="860" y="284" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="edge4" edge="1" parent="1" source="strategy" target="dhaD" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="het_exp" value="&lt;b&gt;异源表达验证&lt;/b&gt;：E. coli BL21表达budC、dhaD，体外酶活测定立体选择性" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#e1d5e7;strokeColor=#9673a6;fontSize=12;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="450" y="406" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="edge5" edge="1" parent="1" source="budA" target="het_exp" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge6" edge="1" parent="1" source="budC" target="het_exp" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge7" edge="1" parent="1" source="dhaD" target="het_exp" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="results" value="&lt;b&gt;关键结果&lt;/b&gt;：葡萄糖时meso为主；甘油时2R,3R-BDO大幅提升；dhaD为2R,3R-BDO唯一来源" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#f8cecc;strokeColor=#b85450;fontSize=12;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="450" y="528" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="edge8" edge="1" parent="1" source="het_exp" target="results" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="conclusion" value="&lt;b&gt;结论&lt;/b&gt;：dhaD（甘油脱氢酶）负责R-乙偶姻→2R,3R-BDO；budC负责双乙酰→S-乙偶姻→2S,3S-BDO；完整途径阐明" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#d5e8d4;strokeColor=#82b366;fontSize=12;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="450" y="650" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="edge9" edge="1" parent="1" source="results" target="conclusion" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
  </root>
</mxGraphModel></diagram></mxfile>