<mxfile host="app.diagrams.net"><diagram name="roadmap"><mxGraphModel dx="1422" dy="794" grid="1" gridSize="10" guides="1" tooltips="1" connect="1" arrows="1" fold="1" page="1" pageScale="1" pageWidth="1169" pageHeight="826" math="0" shadow="0">
  <root>
    <mxCell id="0" />
    <mxCell id="1" parent="0" />
    <mxCell id="node1" value="酿酒酵母异戊醇产量低、副产物多" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#dae8fc;strokeColor=#6c8ebf;fontSize=13;fontStyle=1;absoluteArcSize=12;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="676" y="40" width="236" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node2" value="重建亮氨酸生物合成途径（ILV2/5/3/LEU9/1/2染色体整合）" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#d5e8d4;strokeColor=#82b366;fontSize=12;absoluteArcSize=12;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="40" y="162" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node3" value="过表达Ehrlich途径（ARO10/ADH7质粒表达）" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#d5e8d4;strokeColor=#82b366;fontSize=12;absoluteArcSize=12;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="450" y="162" width="299" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node4" value="过表达线粒体α-IPM转运蛋白OAC1（推拉策略）" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#fff2cc;strokeColor=#d6b656;fontSize=12;absoluteArcSize=12;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="819" y="162" width="320" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node5" value="线粒体区室化减少副产物（异丁醇/2-甲基-1-丁醇）" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#ffe6cc;strokeColor=#d79b00;fontSize=12;absoluteArcSize=12;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="1209" y="162" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node6" value="最优菌株MC5a：异戊醇467.83 mg/L，异丁醇438.59 mg/L" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#f8cecc;strokeColor=#b85450;fontSize=12;absoluteArcSize=12;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="252" y="336" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node7" value="MC5b（+OAC1）：异戊醇561.21 mg/L，异丁醇降至321.64 mg/L" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#f8cecc;strokeColor=#b85450;fontSize=12;absoluteArcSize=12;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="662" y="336" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node8" value="异戊醇提升78倍，转化率14.18 mg/g糖" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#e1d5e7;strokeColor=#9673a6;fontSize=13;fontStyle=1;absoluteArcSize=12;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="1072" y="336" width="264" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node9" value="结论：线粒体区室化+OAC1推导策略有效，奠定代谢工程基础" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#d5e8d4;strokeColor=#82b366;fontSize=13;fontStyle=1;absoluteArcSize=12;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="624" y="510" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="edge1" edge="1" parent="1" source="node1" target="node2" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#6c8ebf;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge2" edge="1" parent="1" source="node1" target="node3" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#6c8ebf;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge3" edge="1" parent="1" source="node1" target="node4" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#6c8ebf;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge4" edge="1" parent="1" source="node2" target="node5" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=1;entryDx=0;entryDy=0;strokeColor=#82b366;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge5" edge="1" parent="1" source="node3" target="node6" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#82b366;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge6" edge="1" parent="1" source="node4" target="node7" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#d6b656;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge7" edge="1" parent="1" source="node5" target="node8" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#9673a6;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge8" edge="1" parent="1" source="node6" target="node8" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=1;entryDx=0;entryDy=0;strokeColor=#b85450;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge9" edge="1" parent="1" source="node7" target="node8" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=1;exitY=0.5;exitDx=0;exitDy=0;entryX=0;entryY=0.5;entryDx=0;entryDy=0;strokeColor=#b85450;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge10" edge="1" parent="1" source="node8" target="node9" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#9673a6;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge11" edge="1" parent="1" source="node1" target="node5" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#999999;dashed=1;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge12" edge="1" parent="1" source="node2" target="node3" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=1;exitY=0.5;exitDx=0;exitDy=0;entryX=0;entryY=0.5;entryDx=0;entryDy=0;strokeColor=#999999;dashed=1;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
  </root>
</mxGraphModel></diagram></mxfile>