<mxfile host="app.diagrams.net"><diagram name="roadmap"><mxGraphModel dx="1200" dy="800" grid="0" gridSize="10" guides="1" tooltips="1" connect="1" arrows="1" fold="1" page="0" pageScale="1" pageWidth="1200" pageHeight="800" math="0" shadow="0" background="#ffffff">
  <root>
    <mxCell id="0" />
    <mxCell id="1" parent="0" />

    
    <mxCell id="node1" value="异源表达产量低&#10;糖基化不均一" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#f8cecc;strokeColor=#b85450;fontSize=12;fontStyle=1;arcSize=10;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="320" y="40" width="210" height="64" as="geometry" />
    </mxCell>

    
    <mxCell id="node2" value="工程化毕赤酵母&#10;SuperMan5菌株表达" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#fff2cc;strokeColor=#d6b656;fontSize=12;fontStyle=1;arcSize=10;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="320" y="162" width="210" height="64" as="geometry" />
    </mxCell>

    
    <mxCell id="node3" value="密码子优化&#10;GbDIR2/GhDIR3" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#d5e8d4;strokeColor=#82b366;fontSize=12;fontStyle=1;arcSize=10;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="40" y="284" width="210" height="64" as="geometry" />
    </mxCell>

    
    <mxCell id="node4" value="补料分批发酵&#10;甲醇流加策略优化" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#d5e8d4;strokeColor=#82b366;fontSize=12;fontStyle=1;arcSize=10;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="320" y="284" width="210" height="64" as="geometry" />
    </mxCell>

    
    <mxCell id="node5" value="亲和纯化+去糖基化&#10;质谱表征糖型" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#d5e8d4;strokeColor=#82b366;fontSize=12;fontStyle=1;arcSize=10;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="600" y="284" width="210" height="64" as="geometry" />
    </mxCell>

    
    <mxCell id="node6" value="产量12/15 mg/L&#10;较植物系统↑60-75倍" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#dae8fc;strokeColor=#6c8ebf;fontSize=12;fontStyle=1;arcSize=10;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="40" y="406" width="210" height="64" as="geometry" />
    </mxCell>

    
    <mxCell id="node7" value="ee达80%/85%&#10;糖基化非活性必需" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#dae8fc;strokeColor=#6c8ebf;fontSize=12;fontStyle=1;arcSize=10;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="320" y="406" width="210" height="64" as="geometry" />
    </mxCell>

    
    <mxCell id="node8" value="N-糖基化位点5-6个&#10;主要糖型Man5GlcNAc2" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#dae8fc;strokeColor=#6c8ebf;fontSize=12;fontStyle=1;arcSize=10;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="600" y="406" width="210" height="64" as="geometry" />
    </mxCell>

    
    <mxCell id="node9" value="高效表达系统建立&#10;结构/机制研究基础" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#e1d5e7;strokeColor=#9673a6;fontSize=12;fontStyle=1;arcSize=10;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="320" y="528" width="210" height="64" as="geometry" />
    </mxCell>

    
    <mxCell id="edge1" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#b85450;fontSize=11;" edge="1" parent="1" source="node1" target="node2">
      <mxGeometry relative="1" as="geometry">
        <mxPoint x="190" y="210" as="sourcePoint" />
        <mxPoint x="280" y="210" as="targetPoint" />
      </mxGeometry>
    </mxCell>

    
    <mxCell id="edge2" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#d6b656;fontSize=11;" edge="1" parent="1" source="node2" target="node3">
      <mxGeometry relative="1" as="geometry">
        <mxPoint x="430" y="190" as="sourcePoint" />
        <mxPoint x="520" y="70" as="targetPoint" />
      </mxGeometry>
    </mxCell>

    
    <mxCell id="edge3" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#d6b656;fontSize=11;" edge="1" parent="1" source="node2" target="node4">
      <mxGeometry relative="1" as="geometry">
        <mxPoint x="430" y="210" as="sourcePoint" />
        <mxPoint x="520" y="160" as="targetPoint" />
      </mxGeometry>
    </mxCell>

    
    <mxCell id="edge4" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#d6b656;fontSize=11;" edge="1" parent="1" source="node2" target="node5">
      <mxGeometry relative="1" as="geometry">
        <mxPoint x="430" y="230" as="sourcePoint" />
        <mxPoint x="520" y="250" as="targetPoint" />
      </mxGeometry>
    </mxCell>

    
    <mxCell id="edge5" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#82b366;fontSize=11;" edge="1" parent="1" source="node3" target="node6">
      <mxGeometry relative="1" as="geometry">
        <mxPoint x="670" y="70" as="sourcePoint" />
        <mxPoint x="760" y="70" as="targetPoint" />
      </mxGeometry>
    </mxCell>

    
    <mxCell id="edge6" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#82b366;fontSize=11;" edge="1" parent="1" source="node4" target="node7">
      <mxGeometry relative="1" as="geometry">
        <mxPoint x="670" y="160" as="sourcePoint" />
        <mxPoint x="760" y="160" as="targetPoint" />
      </mxGeometry>
    </mxCell>

    
    <mxCell id="edge7" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#82b366;fontSize=11;" edge="1" parent="1" source="node5" target="node8">
      <mxGeometry relative="1" as="geometry">
        <mxPoint x="670" y="250" as="sourcePoint" />
        <mxPoint x="760" y="250" as="targetPoint" />
      </mxGeometry>
    </mxCell>

    
    <mxCell id="edge8" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#6c8ebf;fontSize=11;" edge="1" parent="1" source="node6" target="node9">
      <mxGeometry relative="1" as="geometry">
        <mxPoint x="920" y="70" as="sourcePoint" />
        <mxPoint x="1000" y="180" as="targetPoint" />
      </mxGeometry>
    </mxCell>

    
    <mxCell id="edge9" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#6c8ebf;fontSize=11;" edge="1" parent="1" source="node7" target="node9">
      <mxGeometry relative="1" as="geometry">
        <mxPoint x="920" y="160" as="sourcePoint" />
        <mxPoint x="1000" y="210" as="targetPoint" />
      </mxGeometry>
    </mxCell>

    
    <mxCell id="edge10" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#6c8ebf;fontSize=11;" edge="1" parent="1" source="node8" target="node9">
      <mxGeometry relative="1" as="geometry">
        <mxPoint x="920" y="250" as="sourcePoint" />
        <mxPoint x="1000" y="240" as="targetPoint" />
      </mxGeometry>
    </mxCell>

  </root>
</mxGraphModel></diagram></mxfile>