<mxfile host="app.diagrams.net"><diagram name="roadmap"><mxGraphModel dx="1200" dy="800" grid="1" gridSize="10" guides="1" tooltips="1" connect="1" arrows="1" fold="1" page="1" pageScale="1" pageWidth="1000" pageHeight="700" math="0" shadow="0">
  <root>
    <mxCell id="0" />
    <mxCell id="1" parent="0" />
    
    
    <mxCell id="node1" value="研究问题：在3N5M亚家族中寻找高操作稳定胺转氨酶" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#E4F0FF;strokeColor=#4A7FBF;fontSize=12;fontStyle=1;align=center;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="420" y="40" width="340" height="64" as="geometry" />
    </mxCell>
    
    <mxCell id="node2" value="策略：数据库挖掘+指纹筛选，重组表达纯化" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#E8F5E9;strokeColor=#5B9A6B;fontSize=12;fontStyle=1;align=center;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="440" y="214" width="299" height="64" as="geometry" />
    </mxCell>
    
    <mxCell id="node3" value="系统表征：底物谱/储存/操作稳定性/溶剂耐受" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#FFF3E0;strokeColor=#C77A3A;fontSize=12;fontStyle=1;align=center;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="433" y="388" width="313" height="64" as="geometry" />
    </mxCell>
    
    <mxCell id="node4" value="关键改造：TA-10高催化(6 U/mg)+高热稳定(70°C)+溶剂耐受" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#FCE4EC;strokeColor=#B0526A;fontSize=12;fontStyle=1;align=center;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="420" y="562" width="340" height="64" as="geometry" />
    </mxCell>
    
    <mxCell id="node5" value="结果1：TA-10操作16h无失活，60°C/20h仅失活15%" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#F3E5F5;strokeColor=#7A4A9A;fontSize=12;fontStyle=1;align=center;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="40" y="736" width="340" height="64" as="geometry" />
    </mxCell>
    
    <mxCell id="node6" value="结果2：TA-9/TA-1/TA-3N5M稳定性均低于TA-10" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#F3E5F5;strokeColor=#7A4A9A;fontSize=12;fontStyle=1;align=center;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="450" y="736" width="313" height="64" as="geometry" />
    </mxCell>
    
    <mxCell id="node7" value="结果3：二聚体TA-3HMU也稳定，四聚体非必要" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#F3E5F5;strokeColor=#7A4A9A;fontSize=12;fontStyle=1;align=center;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="833" y="736" width="306" height="64" as="geometry" />
    </mxCell>
    
    <mxCell id="node8" value="结论：TA-10是优质生物催化起点；序列指纹是有效发现工具" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#FFEBEE;strokeColor=#C62828;fontSize=12;fontStyle=1;align=center;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="420" y="910" width="340" height="64" as="geometry" />
    </mxCell>
    
    
    <mxCell id="edge1" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#4A7FBF;" edge="1" source="node1" target="node2" parent="1">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    
    <mxCell id="edge2" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#4A7FBF;" edge="1" source="node2" target="node3" parent="1">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    
    <mxCell id="edge3" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#4A7FBF;" edge="1" source="node3" target="node4" parent="1">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    
    <mxCell id="edge4" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#B0526A;dashed=1;" edge="1" source="node4" target="node5" parent="1">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    
    <mxCell id="edge5" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#B0526A;dashed=1;" edge="1" source="node4" target="node6" parent="1">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    
    <mxCell id="edge6" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#B0526A;dashed=1;" edge="1" source="node4" target="node7" parent="1">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    
    <mxCell id="edge7" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#B0526A;" edge="1" source="node5" target="node8" parent="1">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    
    <mxCell id="edge8" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#B0526A;" edge="1" source="node6" target="node8" parent="1">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    
    <mxCell id="edge9" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#B0526A;" edge="1" source="node7" target="node8" parent="1">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
  </root>
</mxGraphModel></diagram></mxfile>