<mxfile host="app.diagrams.net"><diagram name="roadmap"><mxGraphModel dx="1200" dy="800" grid="1" gridSize="10" guides="1" tooltips="1" connect="1" arrows="1" fold="1" page="1" pageScale="1" pageWidth="1600" pageHeight="900" math="0" shadow="0">
  <root>
    <mxCell id="0" />
    <mxCell id="1" parent="0" />
    <mxCell id="node1" value="研究问题：RkACS1与RkACS2在红酵母类胡萝卜素与脂质合成中的不同功能及调控机制" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#DAE8FC;strokeColor=#6C8EBF;fontSize=11;fontStyle=1;align=center;verticalAlign=middle;" vertex="1" parent="1">
      <mxGeometry x="245" y="40" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="edge1" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#6C8EBF;fontSize=10;" edge="1" parent="1" source="node1" target="node2">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="node2" value="策略：CRISPR-Cas9敲除与回补，构建ΔRkACS1、ΔRkACS2及回补菌株" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#D5E8D4;strokeColor=#82B366;fontSize=11;fontStyle=1;align=center;verticalAlign=middle;" vertex="1" parent="1">
      <mxGeometry x="245" y="162" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="edge2" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#82B366;fontSize=10;" edge="1" parent="1" source="node2" target="node3">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="node3" value="表型表征：碳源利用、生长曲线、乙酸/乙酰-CoA/类胡萝卜素/脂质/脂肪酸测定" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#D5E8D4;strokeColor=#82B366;fontSize=11;fontStyle=1;align=center;verticalAlign=middle;" vertex="1" parent="1">
      <mxGeometry x="245" y="284" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="edge3" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#82B366;fontSize=10;" edge="1" parent="1" source="node3" target="node4">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="node4" value="分子分析：qPCR基因表达谱、免疫印迹检测H3K9乙酰化水平" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#FFF2CC;strokeColor=#D6B656;fontSize=11;fontStyle=1;align=center;verticalAlign=middle;" vertex="1" parent="1">
      <mxGeometry x="40" y="406" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="edge4" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#D6B656;fontSize=10;" edge="1" parent="1" source="node4" target="node5">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="node5" value="关键表型：ΔRkACS1类胡萝卜素↓20.2%、总脂↑140.7%、ΔRkACS2总脂↓16.8%、组蛋白乙酰化↓41.2%" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#F8CECC;strokeColor=#B85450;fontSize=11;fontStyle=1;align=center;verticalAlign=middle;" vertex="1" parent="1">
      <mxGeometry x="40" y="528" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="edge5" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#B85450;fontSize=10;" edge="1" parent="1" source="node4" target="node5">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="node6" value="代谢重分布：ΔRkACS1上调FAS1/ACCS（脂质合成），抑制CrtYB/IPI/HMGCR（类胡萝卜素）" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#F8CECC;strokeColor=#B85450;fontSize=11;fontStyle=1;align=center;verticalAlign=middle;" vertex="1" parent="1">
      <mxGeometry x="450" y="406" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="edge6" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#B85450;fontSize=10;" edge="1" parent="1" source="node3" target="node6">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="node7" value="功能冗余与补偿：ΔRkACS1中RkACS2上调9.89倍；回补后类胡萝卜素恢复并超野生型63.6%" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#F8CECC;strokeColor=#B85450;fontSize=11;fontStyle=1;align=center;verticalAlign=middle;" vertex="1" parent="1">
      <mxGeometry x="450" y="528" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="edge7" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#B85450;fontSize=10;" edge="1" parent="1" source="node6" target="node7">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="node8" value="分化机制：RkACS1联系碳源利用与类胡萝卜素合成，RkACS2介导脂质合成及组蛋白乙酰化表观调控" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#E1D5E7;strokeColor=#9673A6;fontSize=11;fontStyle=1;align=center;verticalAlign=middle;" vertex="1" parent="1">
      <mxGeometry x="245" y="650" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="edge8" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#9673A6;fontSize=10;" edge="1" parent="1" source="node7" target="node8">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="node9" value="结论：乙酰-CoA合酶亚型分工为代谢工程靶向改造乙酰-CoA衍生物（类胡萝卜素、脂质）提供理论基础" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#F5F5F5;strokeColor=#666666;fontSize=12;fontStyle=1;align=center;verticalAlign=middle;" vertex="1" parent="1">
      <mxGeometry x="245" y="772" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="edge9" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#666666;fontSize=10;" edge="1" parent="1" source="node8" target="node9">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge10" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=1;exitY=0.5;exitDx=0;exitDy=0;entryX=0;entryY=0.5;entryDx=0;entryDy=0;strokeColor=#B85450;fontSize=10;dashed=1;" edge="1" parent="1" source="node5" target="node7">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge11" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#B85450;fontSize=10;dashed=1;" edge="1" parent="1" source="node5" target="node8">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
  </root>
</mxGraphModel></diagram></mxfile>