<mxfile host="app.diagrams.net"><diagram name="roadmap"><mxGraphModel dx="1200" dy="800" grid="1" gridSize="10" guides="1" tooltips="1" connect="1" arrows="1" fold="1" page="1" pageScale="1" pageWidth="1400" pageHeight="900" math="0" shadow="0">
  <root>
    <mxCell id="0" />
    <mxCell id="1" parent="0" />
    <mxCell id="n1" value="研究问题：解析Nostoc punctiforme PCC 73102中3个酮化酶在光照调控下对canthaxanthin合成的作用" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#DAE8FC;strokeColor=#6C8EBF;fontSize=12;fontStyle=0;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="450" y="40" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="n2" value="策略：大肠杆菌异源表达各酮化酶，结合类胡萝卜素分析及qRT-PCR转录分析" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#D5E8D4;strokeColor=#82B366;fontSize=12;fontStyle=0;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="450" y="162" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="n3" value="关键改造1：crtW148——高光下上调近4倍，唯一催化末端酮化步骤" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#FFF2CC;strokeColor=#D6B656;fontSize=12;fontStyle=0;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="40" y="284" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="n4" value="关键改造2：crtB——高光下上调1.7倍，增加通路上游碳流入" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#FFF2CC;strokeColor=#D6B656;fontSize=12;fontStyle=0;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="450" y="284" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="n5" value="关键改造3：crtW38与crtO——高光下下调，酶活性较低" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#F8CECC;strokeColor=#B85450;fontSize=12;fontStyle=0;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="860" y="284" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="n6" value="结果1：canthaxanthin含量增加约3倍（44→169 μg/g），4-酮基-海胆酮从4→20 μg/g" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#E1D5E7;strokeColor=#9673A6;fontSize=12;fontStyle=0;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="245" y="406" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="n7" value="结果2：phytoene合成速率HL比LL高1.7倍；crtB表达上调1.7倍，crtW148上调近4倍" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#E1D5E7;strokeColor=#9673A6;fontSize=12;fontStyle=0;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="655" y="406" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="n8" value="结论：高光通过转录上调crtB和crtW148增强canthaxanthin合成，CrtW148为HL下主要酮化酶且发挥末端合成作用" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#DAE8FC;strokeColor=#6C8EBF;fontSize=12;fontStyle=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="450" y="528" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="e1" edge="1" parent="1" source="n1" target="n2" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#6C8EBF;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="e2" edge="1" parent="1" source="n2" target="n3" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#D6B656;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="e3" edge="1" parent="1" source="n2" target="n4" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#D6B656;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="e4" edge="1" parent="1" source="n2" target="n5" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#B85450;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="e5" edge="1" parent="1" source="n3" target="n6" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#9673A6;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="e6" edge="1" parent="1" source="n4" target="n7" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#9673A6;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="e7" edge="1" parent="1" source="n5" target="n7" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#B85450;dashed=1;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="e8" edge="1" parent="1" source="n6" target="n8" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#6C8EBF;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="e9" edge="1" parent="1" source="n7" target="n8" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#6C8EBF;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
  </root>
</mxGraphModel></diagram></mxfile>