<mxfile host="app.diagrams.net"><diagram name="roadmap"><mxGraphModel dx="1600" dy="900" grid="1" gridSize="10" guides="1" tooltips="1" connect="1" arrows="1" fold="1" page="1" pageScale="1" pageWidth="1600" pageHeight="900" math="0" shadow="0">
  <root>
    <mxCell id="0" />
    <mxCell id="1" parent="0" />

    
    <mxCell id="node1" value="研究问题：关键酶体内功能未验证" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#dae8fc;strokeColor=#6c8ebf;fontSize=13;fontStyle=1;fontColor=#000000;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="40" y="40" width="236" height="64" as="geometry" />
    </mxCell>

    
    <mxCell id="node2" value="策略：TRV-VIGS 共沉默 GES、ISY、MLPL" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#d5e8d4;strokeColor=#82b366;fontSize=13;fontStyle=1;fontColor=#000000;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="156" y="214" width="278" height="64" as="geometry" />
    </mxCell>

    
    <mxCell id="node3" value="构建 ChlH 可视化标记 VIGS 系统" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#d5e8d4;strokeColor=#82b366;fontSize=13;fontStyle=1;fontColor=#000000;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="486" y="388" width="236" height="64" as="geometry" />
    </mxCell>

    
    <mxCell id="node4" value="共沉默 GES、ISY、MLPL 靶基因" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#ffe6cc;strokeColor=#d79b00;fontSize=13;fontStyle=1;fontColor=#000000;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="493" y="562" width="222" height="64" as="geometry" />
    </mxCell>

    
    <mxCell id="node5" value="GC-MS/LC-MS 结合 qPCR 验证" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#ffe6cc;strokeColor=#d79b00;fontSize=13;fontStyle=1;fontColor=#000000;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="499" y="736" width="210" height="64" as="geometry" />
    </mxCell>

    
    <mxCell id="node6" value="GES 沉默显著降低荆芥内酯" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#f8cecc;strokeColor=#b85450;fontSize=13;fontStyle=1;fontColor=#000000;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="499" y="910" width="210" height="64" as="geometry" />
    </mxCell>

    
    <mxCell id="node7" value="ISY 沉默致前体积累 9 倍" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#f8cecc;strokeColor=#b85450;fontSize=13;fontStyle=1;fontColor=#000000;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="346" y="40" width="210" height="64" as="geometry" />
    </mxCell>

    
    <mxCell id="node8" value="MLPL 仅降 (S)-cis,trans 异构体" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#f8cecc;strokeColor=#b85450;fontSize=13;fontStyle=1;fontColor=#000000;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="626" y="40" width="236" height="64" as="geometry" />
    </mxCell>

    
    <mxCell id="node9" value="1,5,9-epi-DLA 显著下调 1.27 倍" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#f8cecc;strokeColor=#b85450;fontSize=13;fontStyle=1;fontColor=#000000;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="932" y="40" width="236" height="64" as="geometry" />
    </mxCell>

    
    <mxCell id="node10" value="qPCR 确认 mRNA 水平显著降低" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#f8cecc;strokeColor=#b85450;fontSize=13;fontStyle=1;fontColor=#000000;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="496" y="1084" width="215" height="64" as="geometry" />
    </mxCell>

    
    <mxCell id="node11" value="结论：体内验证 GES/ISY/MLPL 功能" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#f5f5f5;strokeColor=#666666;fontSize=13;fontStyle=1;fontColor=#000000;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="504" y="214" width="250" height="64" as="geometry" />
    </mxCell>

    
    <mxCell id="node12" value="局限：NEPS 序列相似性无法验证" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#fff2cc;strokeColor=#d6b656;fontSize=13;fontStyle=0;fontColor=#000000;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="824" y="214" width="229" height="64" as="geometry" />
    </mxCell>

    
    <mxCell id="edge1" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#333333;fontSize=12;" edge="1" parent="1" source="node1" target="node2">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge2" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#333333;fontSize=12;" edge="1" parent="1" source="node2" target="node3">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge3" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#333333;fontSize=12;" edge="1" parent="1" source="node3" target="node4">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge4" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#333333;fontSize=12;" edge="1" parent="1" source="node4" target="node5">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge5" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#333333;fontSize=12;" edge="1" parent="1" source="node5" target="node6">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge6" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#333333;fontSize=12;" edge="1" parent="1" source="node7" target="node11">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>

    
    <mxCell id="edge7" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#333333;fontSize=12;dashed=1;" edge="1" parent="1" source="node6" target="node10">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge8" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0;exitY=0.5;exitDx=0;exitDy=0;entryX=0;entryY=0.5;entryDx=0;entryDy=0;dashed=1;dashed=1;dashed=1;dashed=1;strokeColor=#333333;fontSize=12;dashed=1;" edge="1" parent="1" source="node6" target="node11">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge9" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#333333;fontSize=12;dashed=1;" edge="1" parent="1" source="node7" target="node12">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>

    
    <mxCell id="edge10" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#333333;fontSize=12;" edge="1" parent="1" source="node8" target="node11">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge11" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#333333;fontSize=12;" edge="1" parent="1" source="node9" target="node11">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge12" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0;exitY=0.5;exitDx=0;exitDy=0;entryX=0;entryY=0.5;entryDx=0;entryDy=0;dashed=1;dashed=1;dashed=1;dashed=1;strokeColor=#333333;fontSize=12;" edge="1" parent="1" source="node10" target="node11">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge13" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=1;exitY=0.5;exitDx=0;exitDy=0;entryX=0;entryY=0.5;entryDx=0;entryDy=0;strokeColor=#333333;fontSize=12;" edge="1" parent="1" source="node11" target="node12">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
  </root>
</mxGraphModel></diagram></mxfile>