<mxfile host="app.diagrams.net"><diagram name="roadmap"><mxGraphModel dx="1200" dy="800" grid="1" gridSize="10" guides="1" tooltips="1" connect="1" arrows="1" fold="1" page="1" pageScale="1" pageWidth="1200" pageHeight="800" math="0" shadow="0">
  <root>
    <mxCell id="0" />
    <mxCell id="1" parent="0" />
    
    <mxCell id="node1" vertex="1" parent="1" value="研究问题：M10缬氨霉素产量低，与bafilomycin竞争前体" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#f8cecc;strokeColor=#b85450;fontSize=12;fontColor=#000000;verticalAlign=middle;align=center;">
      <mxGeometry x="655" y="40" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node2" vertex="1" parent="1" value="策略：敲除bafilomycin/BCDH基因，阻断竞争途径，重分配前体流" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#ffe6cc;strokeColor=#d79b00;fontSize=12;fontColor=#000000;verticalAlign=middle;align=center;">
      <mxGeometry x="655" y="162" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node3" vertex="1" parent="1" value="构建4种突变株：ΔbafV、ΔbkdF、ΔbkdA、ΔbkdR" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#fff2cc;strokeColor=#d6b656;fontSize=12;fontColor=#000000;verticalAlign=middle;align=center;">
      <mxGeometry x="655" y="284" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node4" vertex="1" parent="1" value="关键改造1：敲除bafV→bafilomycin消失，缬氨霉素提升1.2-1.5倍" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#d5e8d4;strokeColor=#82b366;fontSize=12;fontColor=#000000;verticalAlign=middle;align=center;">
      <mxGeometry x="40" y="406" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node5" vertex="1" parent="1" value="关键改造2：敲除bkdF→bafilomycin消失，缬氨霉素提升4倍（16 mg/L）" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#d5e8d4;strokeColor=#82b366;fontSize=12;fontColor=#000000;verticalAlign=middle;align=center;">
      <mxGeometry x="450" y="406" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node6" vertex="1" parent="1" value="关键改造3：敲除bkdR→bafilomycin B提升2.4倍（bkdF负调控因子）" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#d5e8d4;strokeColor=#82b366;fontSize=12;fontColor=#000000;verticalAlign=middle;align=center;">
      <mxGeometry x="860" y="406" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node7" vertex="1" parent="1" value="前体饲喂验证：缬氨酸/异亮氨酸/异丁酸介入前体竞争" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#e1d5e7;strokeColor=#9673a6;fontSize=12;fontColor=#000000;verticalAlign=middle;align=center;">
      <mxGeometry x="1270" y="406" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node8" vertex="1" parent="1" value="结果：ΔbkdF缬氨霉素产量最高（16.0 mg/L），抗真菌活性保留" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#dae8fc;strokeColor=#6c8ebf;fontSize=12;fontColor=#000000;verticalAlign=middle;align=center;">
      <mxGeometry x="655" y="528" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node9" vertex="1" parent="1" value="结论：共享前体；敲除bkdF/bafV有效提升缬氨霉素产量" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#dae8fc;strokeColor=#6c8ebf;fontSize=12;fontColor=#000000;verticalAlign=middle;align=center;">
      <mxGeometry x="655" y="650" width="340" height="64" as="geometry" />
    </mxCell>

    <mxCell id="e1" edge="1" parent="1" source="node1" target="node2" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="e2" edge="1" parent="1" source="node2" target="node3" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="e3" edge="1" parent="1" source="node3" target="node4" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="e4" edge="1" parent="1" source="node3" target="node5" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="e5" edge="1" parent="1" source="node3" target="node6" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="e6" edge="1" parent="1" source="node3" target="node7" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="e7" edge="1" parent="1" source="node4" target="node8" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="e8" edge="1" parent="1" source="node5" target="node8" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="e9" edge="1" parent="1" source="node6" target="node8" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="e10" edge="1" parent="1" source="node7" target="node8" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="e11" edge="1" parent="1" source="node8" target="node9" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
  </root>
</mxGraphModel></diagram></mxfile>