<mxfile host="app.diagrams.net"><diagram name="roadmap"><mxGraphModel dx="1422" dy="794" grid="1" gridSize="10" guides="1" tooltips="1" connect="1" arrows="1" fold="1" page="1" pageScale="1" pageWidth="1169" pageHeight="826" math="0" shadow="0">
  <root>
    <mxCell id="0" />
    <mxCell id="1" parent="0" />
    <mxCell id="n1" value="研究问题：在毕赤酵母表面展示活性脂肪酶" vertex="1" parent="1" style="rounded=1;whiteSpace=wrap;html=1;verticalAlign=middle;align=center;">
      <mxGeometry x="44" y="40" width="292" height="64" as="geometry" />
    </mxCell>
    <mxCell id="e1" edge="1" parent="1" source="n1" target="n2" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="n2" value="策略：CALB与Sed1锚定蛋白融合表达" vertex="1" parent="1" style="rounded=1;whiteSpace=wrap;html=1;verticalAlign=middle;align=center;">
      <mxGeometry x="64" y="162" width="250" height="64" as="geometry" />
    </mxCell>
    <mxCell id="e2" edge="1" parent="1" source="n2" target="n3" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="n3" value="关键改造：pAOX1驱动CALBSeD1融合基因构建" vertex="1" parent="1" style="rounded=1;whiteSpace=wrap;html=1;verticalAlign=middle;align=center;">
      <mxGeometry x="40" y="284" width="299" height="64" as="geometry" />
    </mxCell>
    <mxCell id="e3" edge="1" parent="1" source="n3" target="n4" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="n4" value="结果1：展示酶活性超220 U/g干细胞" vertex="1" parent="1" style="rounded=1;whiteSpace=wrap;html=1;verticalAlign=middle;align=center;">
      <mxGeometry x="64" y="406" width="250" height="64" as="geometry" />
    </mxCell>
    <mxCell id="e4" edge="1" parent="1" source="n4" target="n5" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="n5" value="结果2：60°C热稳定性显著优于游离酶" vertex="1" parent="1" style="rounded=1;whiteSpace=wrap;html=1;verticalAlign=middle;align=center;">
      <mxGeometry x="58" y="528" width="264" height="64" as="geometry" />
    </mxCell>
    <mxCell id="e5" edge="1" parent="1" source="n5" target="n6" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="n6" value="结论：构建全细胞催化剂替代游离脂肪酶" vertex="1" parent="1" style="rounded=1;whiteSpace=wrap;html=1;verticalAlign=middle;align=center;">
      <mxGeometry x="50" y="650" width="278" height="64" as="geometry" />
    </mxCell>
  </root>
</mxGraphModel></diagram></mxfile>