<mxfile host="app.diagrams.net"><diagram name="roadmap"><mxGraphModel dx="1200" dy="800" grid="1" gridSize="10" guides="1" tooltips="1" connect="1" arrows="1" fold="1" page="1" pageScale="1" pageWidth="1200" pageHeight="800">
  <root>
    <mxCell id="0" />
    <mxCell id="1" parent="0" />

    
    <mxCell id="n1" value="研究问题：鉴定催化亚麻酸13位氢过氧化的LOX基因" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#dae8fc;strokeColor=#6c8ebf;fontFamily=SimSun;fontSize=13;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="211" y="40" width="340" height="64" as="geometry" />
    </mxCell>

    <mxCell id="n2" value="策略：筛选EST库中25个LOX候选基因" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#d5e8d4;strokeColor=#82b366;fontFamily=SimSun;fontSize=13;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="256" y="214" width="250" height="64" as="geometry" />
    </mxCell>

    <mxCell id="n3" value="策略：RTqPCR比较表达模式，选取TcCDS相似者" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#d5e8d4;strokeColor=#82b366;fontFamily=SimSun;fontSize=13;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="224" y="388" width="313" height="64" as="geometry" />
    </mxCell>

    <mxCell id="n4" value="关键改造：RACE克隆TcLOX1/TcLOX2全长cDNA" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#ffe6cc;strokeColor=#d79b00;fontFamily=SimSun;fontSize=13;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="232" y="562" width="299" height="64" as="geometry" />
    </mxCell>

    <mxCell id="n5" value="关键改造：大肠杆菌异源表达重组蛋白" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#ffe6cc;strokeColor=#d79b00;fontFamily=SimSun;fontSize=13;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="249" y="736" width="264" height="64" as="geometry" />
    </mxCell>

    <mxCell id="n6" value="结果：TcLOX1催化生成13-HPOT，TcLOX2无活性" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#f8cecc;strokeColor=#b85450;fontFamily=SimSun;fontSize=13;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="40" y="910" width="313" height="64" as="geometry" />
    </mxCell>

    <mxCell id="n7" value="结果：TcLOX1毛状体高表达，与TcCDS共表达" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#f8cecc;strokeColor=#b85450;fontFamily=SimSun;fontSize=13;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="423" y="910" width="299" height="64" as="geometry" />
    </mxCell>

    <mxCell id="n8" value="结论：TcLOX1是除虫菊酯醇部分第一步的关键酶" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#e1d5e7;strokeColor=#9673a6;fontFamily=SimSun;fontSize=13;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="221" y="1084" width="320" height="64" as="geometry" />
    </mxCell>

    
    <mxCell id="e1" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#333333;" edge="1" parent="1" source="n1" target="n2">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>

    <mxCell id="e2" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#333333;" edge="1" parent="1" source="n2" target="n3">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>

    <mxCell id="e3" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#333333;" edge="1" parent="1" source="n3" target="n4">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>

    <mxCell id="e4" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#333333;" edge="1" parent="1" source="n4" target="n5">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>

    <mxCell id="e5" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#b85450;" edge="1" parent="1" source="n5" target="n6">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>

    <mxCell id="e6" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#333333;" edge="1" parent="1" source="n5" target="n7">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>

    <mxCell id="e7" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#333333;" edge="1" parent="1" source="n7" target="n8">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>

    <mxCell id="e8" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0;exitY=0.5;exitDx=0;exitDy=0;entryX=0;entryY=0.5;entryDx=0;entryDy=0;dashed=1;strokeColor=#333333;" edge="1" parent="1" source="n8" target="n6">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>

  </root>
</mxGraphModel></diagram></mxfile>