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    <mxCell id="node2" value="策略：三轮“发酵-筛选-转接”适应性进化，每轮500小时CCCF培养，以恶化发酵液为选择压力" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#d5e8d4;strokeColor=#82b366;fontSize=12;fontStyle=1;align=center;verticalAlign=middle;arcSize=8;" vertex="1" parent="1">
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    <mxCell id="node3" value="关键改造1：首次将CCCF与PDMS膜渗透汽化耦合，膜分离缓解乙醇抑制并积累不可渗透代谢物形成独特选择压力" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#fff2cc;strokeColor=#d6b656;fontSize=12;fontStyle=1;align=center;verticalAlign=middle;arcSize=8;" vertex="1" parent="1">
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    <mxCell id="node4" value="关键改造2：无需基因改造，三轮连续筛选获得600代后代菌株S33（原始ADY菌株经自然选择进化）" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#fff2cc;strokeColor=#d6b656;fontSize=12;fontStyle=1;align=center;verticalAlign=middle;arcSize=8;" vertex="1" parent="1">
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    <mxCell id="node5" value="结果1：S33在残液培养基摇瓶中乙醇产量和比乙醇生产率较亲本ADY分别提高34.5%和34.7%" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#f8cecc;strokeColor=#b85450;fontSize=12;fontStyle=0;align=center;verticalAlign=middle;arcSize=8;" vertex="1" parent="1">
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    <mxCell id="node6" value="结果2：第三轮样品在NM培养中平均比葡萄糖利用率(0.97 h⁻¹)高于前两轮(0.70, 0.63 h⁻¹)，但平均乙醇产量(0.34 g/g)低于前两轮(0.44, 0.42 g/g)" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#f8cecc;strokeColor=#b85450;fontSize=12;fontStyle=0;align=center;verticalAlign=middle;arcSize=8;" vertex="1" parent="1">
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    <mxCell id="node7" value="结果3：第三代株比乙醇生产率(0.33 h⁻¹)高于前两代(0.31, 0.26 h⁻¹)，终发酵液乙醇1.03%、甘油5.7%，平均细胞规模4.06×10⁸ cells/mL" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#f8cecc;strokeColor=#b85450;fontSize=12;fontStyle=0;align=center;verticalAlign=middle;arcSize=8;" vertex="1" parent="1">
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    <mxCell id="node8" value="结论：长期CCCF系统（PDMS膜渗透汽化耦合）是有效的适应性进化方法，600代后代菌株发酵性能显著优于亲本，可推广至其他产乙醇微生物" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#dae8fc;strokeColor=#6c8ebf;fontSize=12;fontStyle=1;align=center;verticalAlign=middle;arcSize=8;" vertex="1" parent="1">
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