<mxfile host="app.diagrams.net"><diagram name="roadmap"><mxGraphModel dx="1200" dy="800" grid="1" gridSize="10" guides="1" tooltips="1" connect="1" arrows="1" fold="1" page="1" pageScale="1" pageWidth="1200" pageHeight="800" math="0" shadow="0">
  <root>
    <mxCell id="0" />
    <mxCell id="1" parent="0" />

    
    <mxCell id="node1" value="研究问题：鉴定黑胡椒未成熟果实中风味倍半萜合酶基因" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#dae8fc;strokeColor=#6c8ebf;fontSize=12;fontStyle=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="437" y="40" width="340" height="64" as="geometry" />
    </mxCell>

    <mxCell id="node2" value="策略：转录组挖掘+异源表达+功能鉴定" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#d5e8d4;strokeColor=#82b366;fontSize=12;fontStyle=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="475" y="214" width="264" height="64" as="geometry" />
    </mxCell>

    <mxCell id="node3" value="关键改造：克隆3个全长TPS cDNAs" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#ffe6cc;strokeColor=#d79b00;fontSize=12;fontStyle=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="489" y="388" width="236" height="64" as="geometry" />
    </mxCell>

    <mxCell id="node4" value="PnTPS1（石竹烯合酶）体外主产物β-石竹烯97%" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#f8cecc;strokeColor=#b85450;fontSize=11;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="40" y="562" width="320" height="64" as="geometry" />
    </mxCell>

    <mxCell id="node5" value="PnTPS2（杜松醇合酶）体外主产物δ-cadinol 91%" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#f8cecc;strokeColor=#b85450;fontSize=11;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="430" y="562" width="334" height="64" as="geometry" />
    </mxCell>

    <mxCell id="node6" value="PnTPS3（大牻牛儿烯D合酶）体外产物germacrene D 91%" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#f8cecc;strokeColor=#b85450;fontSize=11;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="834" y="562" width="340" height="64" as="geometry" />
    </mxCell>

    <mxCell id="node7" value="体内证据：PnGDS酸性重排生成copaene和β-cadinene" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#fff2cc;strokeColor=#d6b656;fontSize=11;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="625" y="736" width="340" height="64" as="geometry" />
    </mxCell>

    <mxCell id="node8" value="结果：未成熟果实中β-石竹烯占倍半萜65.8%" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#ddd6f3;strokeColor=#9673a6;fontSize=12;fontStyle=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="249" y="736" width="306" height="64" as="geometry" />
    </mxCell>

    <mxCell id="node9" value="结论：3个TPS负责黑胡椒主要风味倍半萜合成" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#dae8fc;strokeColor=#6c8ebf;fontSize=12;fontStyle=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="454" y="910" width="306" height="64" as="geometry" />
    </mxCell>

    
    <mxCell id="edge1" edge="1" parent="1" source="node1" target="node2" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#6c8ebf;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>

    <mxCell id="edge2" edge="1" parent="1" source="node2" target="node3" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#6c8ebf;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>

    <mxCell id="edge3" edge="1" parent="1" source="node3" target="node4" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#6c8ebf;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>

    <mxCell id="edge4" edge="1" parent="1" source="node3" target="node5" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#6c8ebf;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>

    <mxCell id="edge5" edge="1" parent="1" source="node3" target="node6" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#6c8ebf;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>

    <mxCell id="edge6" edge="1" parent="1" source="node4" target="node8" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#6c8ebf;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>

    <mxCell id="edge7" edge="1" parent="1" source="node5" target="node8" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#6c8ebf;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>

    <mxCell id="edge8" edge="1" parent="1" source="node6" target="node7" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#6c8ebf;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>

    <mxCell id="edge9" edge="1" parent="1" source="node7" target="node9" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#6c8ebf;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>

    <mxCell id="edge10" edge="1" parent="1" source="node8" target="node9" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#6c8ebf;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>

  </root>
</mxGraphModel></diagram></mxfile>