<mxfile host="app.diagrams.net"><diagram name="roadmap"><mxGraphModel dx="1200" dy="800" grid="1" gridSize="10" guide="1" tooltips="1" connect="1" arrows="1" fold="1" page="1" pageScale="1" pageWidth="1400" pageHeight="900" math="0" shadow="0">
  <root>
    <mxCell id="0" />
    <mxCell id="1" parent="0" />
    
    
    <mxCell id="node1" value="明确紫杉醇C13侧链2'-羟基化反应时机" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#dae8fc;strokeColor=#6c8ebf;fontSize=14;fontStyle=1;verticalAlign=middle;align=center;spacing=5;arcSize=10;" vertex="1" parent="1">
      <mxGeometry x="381" y="40" width="264" height="64" as="geometry" />
    </mxCell>
    
    
    <mxCell id="node2" value="无细胞酶系统（可溶部分+微粒体）" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#d5e8d4;strokeColor=#82b366;fontSize=13;verticalAlign=middle;align=center;spacing=5;arcSize=10;" vertex="1" parent="1">
      <mxGeometry x="392" y="162" width="243" height="64" as="geometry" />
    </mxCell>
    
    
    <mxCell id="node3" value="三个放射性标记底物进行酶活检测" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#ffe6cc;strokeColor=#d79b00;fontSize=13;verticalAlign=middle;align=center;spacing=5;arcSize=10;" vertex="1" parent="1">
      <mxGeometry x="395" y="284" width="236" height="64" as="geometry" />
    </mxCell>
    
    
    <mxCell id="node4" value="N-去苯甲酰-2'-脱氧紫杉醇被羟基化" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#f8cecc;strokeColor=#b85450;fontSize=13;fontStyle=1;verticalAlign=middle;align=center;spacing=5;arcSize=10;" vertex="1" parent="1">
      <mxGeometry x="388" y="406" width="250" height="64" as="geometry" />
    </mxCell>
    
    
    <mxCell id="node5" value="依赖O2/NADPH/FAD/FMN，被CO和唑类抑制" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#e1d5e7;strokeColor=#9673a6;fontSize=13;verticalAlign=middle;align=center;spacing=5;arcSize=10;" vertex="1" parent="1">
      <mxGeometry x="40" y="528" width="278" height="64" as="geometry" />
    </mxCell>
    
    
    <mxCell id="node6" value="β-苯丙氨酸和2'-脱氧紫杉醇不被羟化" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#fff2cc;strokeColor=#d6b656;fontSize=13;verticalAlign=middle;align=center;spacing=5;arcSize=10;" vertex="1" parent="1">
      <mxGeometry x="388" y="528" width="264" height="64" as="geometry" />
    </mxCell>
    
    
    <mxCell id="node7" value="侧链转移后、N-苯甲酰化前发生羟基化" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#dae8fc;strokeColor=#6c8ebf;fontSize=14;fontStyle=1;verticalAlign=middle;align=center;spacing=5;arcSize=10;" vertex="1" parent="1">
      <mxGeometry x="722" y="528" width="264" height="64" as="geometry" />
    </mxCell>
    
    
    <mxCell id="node8" value="明确PAM→CoA连接酶→C13转移酶→P450羟化酶→N-苯甲酰转移酶顺序" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#d5e8d4;strokeColor=#82b366;fontSize=13;verticalAlign=middle;align=center;spacing=5;arcSize=10;" vertex="1" parent="1">
      <mxGeometry x="538" y="650" width="340" height="64" as="geometry" />
    </mxCell>
    
    
    <mxCell id="node9" value="定位微粒体P450 2'-羟化酶，奠定基因克隆基础" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#f8cecc;strokeColor=#b85450;fontSize=14;fontStyle=1;verticalAlign=middle;align=center;spacing=5;arcSize=10;" vertex="1" parent="1">
      <mxGeometry x="148" y="650" width="320" height="64" as="geometry" />
    </mxCell>
    
    
    <mxCell id="edge1" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#6c8ebf;" edge="1" source="node1" target="node2" parent="1">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge2" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#d79b00;" edge="1" source="node2" target="node3" parent="1">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge3" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#b85450;" edge="1" source="node3" target="node4" parent="1">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge4" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#b85450;" edge="1" source="node4" target="node5" parent="1">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge5" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#d6b656;" edge="1" source="node4" target="node6" parent="1">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge6" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#6c8ebf;" edge="1" source="node4" target="node7" parent="1">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge7" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#82b366;dashed=1;" edge="1" source="node7" target="node8" parent="1">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge8" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#b85450;" edge="1" source="node7" target="node9" parent="1">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge9" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#9673a6;dashed=1;" edge="1" source="node5" target="node9" parent="1">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
  </root>
</mxGraphModel></diagram></mxfile>