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    <mxCell id="node2" value="策略：从近平滑念珠菌纯化SCRII→克隆基因→大肠杆菌过表达→定点突变" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#d5e8d4;strokeColor=#82b366;fontSize=12;fontStyle=1;verticalAlign=middle;align=center;fontColor=#1a1a1a;shadow=1;arcSize=10;" vertex="1" parent="1">
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    <mxCell id="node3" value="关键改造：定点突变N142、A220、E228（SDR家族识别与催化残基）" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#fff2cc;strokeColor=#d6b656;fontSize=12;fontStyle=1;verticalAlign=middle;align=center;fontColor=#1a1a1a;shadow=1;arcSize=10;" vertex="1" parent="1">
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    <mxCell id="node4" value="结果①：野生型SCRII催化2-羟基苯乙酮→(S)-PED，光学纯度100%，收率98.1%（比活力5.98 U/mg）" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#f8cecc;strokeColor=#b85450;fontSize=11;verticalAlign=middle;align=center;fontColor=#1a1a1a;shadow=1;arcSize=10;" vertex="1" parent="1">
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    <mxCell id="node5" value="结果②：A220/E228突变体比活力↓20倍，催化效率&lt;野生型7%，对映选择性几乎丧失；N142突变无显著影响" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#f8cecc;strokeColor=#b85450;fontSize=11;verticalAlign=middle;align=center;fontColor=#1a1a1a;shadow=1;arcSize=10;" vertex="1" parent="1">
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    <mxCell id="node6" value="结果③：最适温度35°C，最适pH 6.0，40°C及pH5-7稳定性良好（工业应用潜力）" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#f8cecc;strokeColor=#b85450;fontSize=11;verticalAlign=middle;align=center;fontColor=#1a1a1a;shadow=1;arcSize=10;" vertex="1" parent="1">
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    <mxCell id="node7" value="结论：SCRII为抗Prelog(S)-特异性还原酶，绝对立体选择性+高收率，A220/E228为底物结合与催化关键残基，适用于不对称合成与SDR结构-功能研究" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#e1d5e7;strokeColor=#9673a6;fontSize=12;fontStyle=1;verticalAlign=middle;align=center;fontColor=#1a1a1a;shadow=1;arcSize=10;" vertex="1" parent="1">
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