<mxfile host="app.diagrams.net"><diagram name="roadmap"><mxGraphModel dx="1600" dy="800" grid="1" gridSize="10" guides="1" tooltips="1" connect="1" arrows="1" fold="1" page="1" pageScale="1" pageWidth="1400" pageHeight="900" math="0" shadow="0">
  <root>
    <mxCell id="0" />
    <mxCell id="1" parent="0" />
    <mxCell id="node1" value="核心问题：葡萄糖碳源上G. oxydans生长差，高甘油下DHA产量受限" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#dae8fc;strokeColor=#6c8ebf;fontSize=12;fontStyle=1;align=center;verticalAlign=middle;" vertex="1" parent="1">
      <mxGeometry x="245" y="40" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node2" value="策略：代谢工程+适应性进化，葡萄糖为唯一碳源" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#d5e8d4;strokeColor=#82b366;fontSize=12;fontStyle=1;align=center;verticalAlign=middle;" vertex="1" parent="1">
      <mxGeometry x="252" y="162" width="327" height="64" as="geometry" />
    </mxCell>
    <mxCell id="edge1" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#333333;" edge="1" parent="1" source="node1" target="node2">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="node3" value="敲除adh，过表达sldAB，缺失mgdh，搭建工程菌骨架" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#ffe6cc;strokeColor=#d79b00;fontSize=12;align=center;verticalAlign=middle;" vertex="1" parent="1">
      <mxGeometry x="245" y="284" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="edge2" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#333333;" edge="1" parent="1" source="node2" target="node3">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="node4" value="适应性进化9个月，获得高效葡萄糖利用突变株GAT，sldAB表达提升10倍" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#ffe6cc;strokeColor=#d79b00;fontSize=12;align=center;verticalAlign=middle;" vertex="1" parent="1">
      <mxGeometry x="245" y="406" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="edge3" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#333333;" edge="1" parent="1" source="node3" target="node4">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="node5" value="GN菌株：最大比生长速率提升至0.28-0.29/h，葡萄糖生物量产率提高10倍" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#f8cecc;strokeColor=#b85450;fontSize=12;align=center;verticalAlign=middle;" vertex="1" parent="1">
      <mxGeometry x="40" y="528" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="edge4" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#333333;" edge="1" parent="1" source="node4" target="node5">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="node6" value="葡萄糖培养促进sldAB高表达，Km降至0.9g/L，催化活性提高30-40%" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#f8cecc;strokeColor=#b85450;fontSize=12;align=center;verticalAlign=middle;" vertex="1" parent="1">
      <mxGeometry x="450" y="528" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="edge5" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#333333;dashed=1;" edge="1" parent="1" source="node4" target="node6">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="node7" value="40g/L甘油时DHA产率99.2%（4.7g/gCDW/h），较对照组提高86%；100g/L时产量达88.1g/L" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#f8cecc;strokeColor=#b85450;fontSize=12;align=center;verticalAlign=middle;" vertex="1" parent="1">
      <mxGeometry x="40" y="650" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="edge6" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#333333;" edge="1" parent="1" source="node5" target="node7">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge7" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#333333;" edge="1" parent="1" source="node6" target="node7">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="node8" value="结论：葡萄糖为碳源既降本又提升催化能力，为工业化DHA生产提供有效策略" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#e1d5e7;strokeColor=#9673a6;fontSize=12;fontStyle=1;align=center;verticalAlign=middle;" vertex="1" parent="1">
      <mxGeometry x="450" y="650" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="edge8" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=1;exitY=0.5;exitDx=0;exitDy=0;entryX=0;entryY=0.5;entryDx=0;entryDy=0;strokeColor=#333333;" edge="1" parent="1" source="node7" target="node8">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge9" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#333333;" edge="1" parent="1" source="node5" target="node8">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge10" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#333333;dashed=1;" edge="1" parent="1" source="node6" target="node8">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
  </root>
</mxGraphModel></diagram></mxfile>