<mxfile host="app.diagrams.net"><diagram name="roadmap"><mxGraphModel dx="1422" dy="793" grid="1" gridSize="10" guides="1" tooltips="1" connect="1" arrows="1" fold="1" page="1" pageScale="1" pageWidth="1400" pageHeight="900" math="0" shadow="0">
  <root>
    <mxCell id="0" />
    <mxCell id="1" parent="0" />
    
    
    <mxCell id="n1" value="研究问题：BGL催化纤维二糖水解为限速步骤，且受葡萄糖反馈抑制" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#dae8fc;strokeColor=#6c8ebf;fontSize=11;fontStyle=1;verticalAlign=middle;align=center;shadow=0;arcSize=8;" vertex="1" parent="1">
      <mxGeometry x="238" y="40" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="n2" value="策略：系统综述BGL分类、微生物生产及异源表达策略" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#d5e8d4;strokeColor=#82b366;fontSize=11;fontStyle=1;verticalAlign=middle;align=center;shadow=0;arcSize=8;" vertex="1" parent="1">
      <mxGeometry x="238" y="162" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="n3" value="关键改造1：寻找葡萄糖耐受型BGL并补充至纤维素酶系" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#fff2cc;strokeColor=#d6b656;fontSize=11;fontStyle=1;verticalAlign=middle;align=center;shadow=0;arcSize=8;" vertex="1" parent="1">
      <mxGeometry x="238" y="284" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="n4" value="关键改造2：重组DNA技术使工业菌株过量表达BGL" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#fff2cc;strokeColor=#d6b656;fontSize=11;fontStyle=1;verticalAlign=middle;align=center;shadow=0;arcSize=8;" vertex="1" parent="1">
      <mxGeometry x="40" y="406" width="327" height="64" as="geometry" />
    </mxCell>
    <mxCell id="n5" value="关键改造3：构建多酶复合体表面展示协同作用" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#fff2cc;strokeColor=#d6b656;fontSize=11;fontStyle=1;verticalAlign=middle;align=center;shadow=0;arcSize=8;" vertex="1" parent="1">
      <mxGeometry x="47" y="528" width="313" height="64" as="geometry" />
    </mxCell>
    <mxCell id="n6" value="结果：BGL活性大幅提升（最高63倍），耐受性增强，乙醇产率达88%" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#f8cecc;strokeColor=#b85450;fontSize=11;fontStyle=1;verticalAlign=middle;align=center;shadow=0;arcSize=8;" vertex="1" parent="1">
      <mxGeometry x="437" y="406" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="n7" value="结论：开发葡萄糖耐受型BGL及基因工程改造宿主是提高纤维素水解效率的有效途径" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#f5f5f5;strokeColor=#666666;fontSize=11;fontStyle=2;verticalAlign=middle;align=center;shadow=0;arcSize=8;" vertex="1" parent="1">
      <mxGeometry x="430" y="528" width="340" height="64" as="geometry" />
    </mxCell>

    
    <mxCell id="e1" edge="1" parent="1" source="n1" target="n2" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#6c8ebf;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="e2" edge="1" parent="1" source="n2" target="n3" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#6c8ebf;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="e3" edge="1" parent="1" source="n3" target="n4" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#6c8ebf;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="e4" edge="1" parent="1" source="n4" target="n5" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#6c8ebf;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="e5" edge="1" parent="1" source="n3" target="n6" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#82b366;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="e6" edge="1" parent="1" source="n4" target="n6" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=1;exitY=0.5;exitDx=0;exitDy=0;entryX=0;entryY=0.5;entryDx=0;entryDy=0;strokeColor=#82b366;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="e7" edge="1" parent="1" source="n5" target="n6" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0;exitY=0.5;exitDx=0;exitDy=0;entryX=0;entryY=0.5;entryDx=0;entryDy=0;dashed=1;dashed=1;dashed=1;dashed=1;strokeColor=#82b366;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="e8" edge="1" parent="1" source="n6" target="n7" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#b85450;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
  </root>
</mxGraphModel></diagram></mxfile>