<mxfile host="app.diagrams.net"><diagram name="roadmap"><mxGraphModel dx="1422" dy="762" grid="1" gridSize="10" guides="1" tooltips="1" connect="1" arrows="1" fold="1" page="1" pageScale="1" pageWidth="1400" pageHeight="900" math="0" shadow="0">
  <root>
    <mxCell id="0" />
    <mxCell id="1" parent="0" />
    <mxCell id="research_problem" value="真菌次级代谢产物活性强但化学合成困难，基因簇表达差，产量不足" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#f8cecc;strokeColor=#b85450;fontSize=12;fontStyle=1;verticalAlign=middle;align=center;width=180;height=60;" vertex="1" parent="1">
      <mxGeometry x="450" y="40" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="strategy_objective" value="策略：以FTM-C和terpendole E为目标，运用路径工程改造与沉默基因簇激活策略" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#fff2cc;strokeColor=#d6b656;fontSize=12;fontStyle=1;verticalAlign=middle;align=center;width=180;height=60;" vertex="1" parent="1">
      <mxGeometry x="450" y="162" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="pathway_fumitremorgin" value="改造fumitremorgin途径：确认FtmE催化C环环化生成FTM-C(BCRP强抑制)" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#dae8fc;strokeColor=#6c8ebf;fontSize=11;verticalAlign=middle;align=center;width=180;height=60;" vertex="1" parent="1">
      <mxGeometry x="40" y="284" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="silent_cluster_activation" value="激活沉默基因簇：干扰双组分信号系统，鉴定TAS1(NRPS-PKS杂合酶)合成TeA" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#dae8fc;strokeColor=#6c8ebf;fontSize=11;verticalAlign=middle;align=center;width=180;height=60;" vertex="1" parent="1">
      <mxGeometry x="450" y="284" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="pathway_terpendole" value="改造terpendole途径：阐明TerQ/TerP功能，敲除terP阻断分流，实现稳定过量生产" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#dae8fc;strokeColor=#6c8ebf;fontSize=11;verticalAlign=middle;align=center;width=180;height=60;" vertex="1" parent="1">
      <mxGeometry x="860" y="284" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="result_compounds" value="结果：获得FTM系列BCRP抑制剂、terpendole E及其新类似物11-酮基paspaline、TeA" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#d5e8d4;strokeColor=#82b366;fontSize=12;fontStyle=1;verticalAlign=middle;align=center;width=180;height=60;" vertex="1" parent="1">
      <mxGeometry x="450" y="406" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="activity_evaluation" value="活性评价：11-酮基paspaline对Eg5 ATPase抑制IC50=16.5μM，优于terpendole E(C11酮基关键)" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#d5e8d4;strokeColor=#82b366;fontSize=11;verticalAlign=middle;align=center;width=200;height=60;" vertex="1" parent="1">
      <mxGeometry x="245" y="528" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="conclusion" value="结论：途径工程+激活沉默代谢+新酶应用=高效制备复杂天然产物与药物先导发现" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#e1d5e7;strokeColor=#9673a6;fontSize=12;fontStyle=1;verticalAlign=middle;align=center;width=200;height=70;" vertex="1" parent="1">
      <mxGeometry x="655" y="528" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="e1" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#b85450;" edge="1" parent="1" source="research_problem" target="strategy_objective">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="e2" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#6c8ebf;" edge="1" parent="1" source="strategy_objective" target="pathway_fumitremorgin">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="e3" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#6c8ebf;" edge="1" parent="1" source="strategy_objective" target="silent_cluster_activation">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="e4" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#6c8ebf;" edge="1" parent="1" source="strategy_objective" target="pathway_terpendole">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="e5" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#82b366;" edge="1" parent="1" source="pathway_fumitremorgin" target="result_compounds">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="e6" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#82b366;" edge="1" parent="1" source="silent_cluster_activation" target="result_compounds">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="e7" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#82b366;" edge="1" parent="1" source="pathway_terpendole" target="result_compounds">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="e8" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#82b366;" edge="1" parent="1" source="result_compounds" target="activity_evaluation">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="e9" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=1;exitY=0.5;exitDx=0;exitDy=0;entryX=0;entryY=0.5;entryDx=0;entryDy=0;strokeColor=#9673a6;" edge="1" parent="1" source="activity_evaluation" target="conclusion">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="e10" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#9673a6;dashed=1;" edge="1" parent="1" source="result_compounds" target="conclusion">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
  </root>
</mxGraphModel></diagram></mxfile>