<mxfile host="app.diagrams.net"><diagram name="roadmap"><mxGraphModel dx="1200" dy="800" grid="1" gridSize="10" guides="1" tooltips="1" connect="1" arrows="1" fold="1" page="1" pageScale="1" pageWidth="1400" pageHeight="900" math="0" shadow="0">
  <root>
    <mxCell id="0" />
    <mxCell id="1" parent="0" />
    <mxCell id="node1" value="核心问题：酿酒酵母缺乏降解生淀粉能力，传统乙醇生产需高温液化和酶解等昂贵预处理" style="shape=roundedRect;whiteSpace=wrap;html=1;fillColor=#FFE6CC;strokeColor=#E67E22;fontSize=13;fontStyle=1;verticalAlign=middle;perimeter=rectanglePoints;" vertex="1" parent="1">
      <mxGeometry x="245" y="40" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="edge1" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#E67E22;" edge="1" parent="1" source="node1" target="node2">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="node2" value="表达α-淀粉酶和葡糖淀粉酶的四倍体酿酒酵母菌株MNIV/6GS" style="shape=roundedRect;whiteSpace=wrap;html=1;fillColor=#D5E8D4;strokeColor=#82B366;fontSize=13;fontStyle=1;verticalAlign=middle;perimeter=rectanglePoints;" vertex="1" parent="1">
      <mxGeometry x="40" y="162" width="340" height="73" as="geometry" />
    </mxCell>
    <mxCell id="edge2" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#82B366;" edge="1" parent="1" source="node2" target="node3">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="node3" value="创新点：首次直接从高产水稻（真实生物质）生产乙醇，不需预处理和商业酶；多倍化提高鲁棒性；水稻自身满足营养需求" style="shape=roundedRect;whiteSpace=wrap;html=1;fillColor=#DAE8FC;strokeColor=#6C8EBF;fontSize=13;fontStyle=1;verticalAlign=middle;perimeter=rectanglePoints;" vertex="1" parent="1">
      <mxGeometry x="245" y="293" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="edge3" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#6C8EBF;" edge="1" parent="1" source="node3" target="node4">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="node4" value="关键发现：有营养补充下，褐米乙醇生产率1.1 g/L/h（约玉米5倍），产率96%" style="shape=roundedRect;whiteSpace=wrap;html=1;fillColor=#F8CECC;strokeColor=#B85450;fontSize=13;fontStyle=1;verticalAlign=middle;perimeter=rectanglePoints;" vertex="1" parent="1">
      <mxGeometry x="245" y="415" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="edge4" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#B85450;" edge="1" parent="1" source="node4" target="node5">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="node5" value="无营养补充时：10%接种量产率101%，20%接种量生产率达1.8 g/L/h" style="shape=roundedRect;whiteSpace=wrap;html=1;fillColor=#F8CECC;strokeColor=#B85450;fontSize=13;fontStyle=1;verticalAlign=middle;perimeter=rectanglePoints;" vertex="1" parent="1">
      <mxGeometry x="245" y="537" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="edge5" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#B85450;" edge="1" parent="1" source="node5" target="node6">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="node6" value="2L发酵罐放大：10%接种量生产率1.2 g/L/h，理论产率101%" style="shape=roundedRect;whiteSpace=wrap;html=1;fillColor=#F8CECC;strokeColor=#B85450;fontSize=13;fontStyle=1;verticalAlign=middle;perimeter=rectanglePoints;" vertex="1" parent="1">
      <mxGeometry x="245" y="659" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="edge6" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#B85450;" edge="1" parent="1" source="node6" target="node7">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="node7" value="酶活性：褐米发酵中葡糖淀粉酶16.3 U/mL、α-淀粉酶22.6 U/mL（远超玉米10倍以上）" style="shape=roundedRect;whiteSpace=wrap;html=1;fillColor=#F8CECC;strokeColor=#B85450;fontSize=13;fontStyle=1;verticalAlign=middle;perimeter=rectanglePoints;" vertex="1" parent="1">
      <mxGeometry x="245" y="781" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="edge7" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0;exitY=0.5;exitDx=0;exitDy=0;entryX=0;entryY=0.5;entryDx=0;entryDy=0;dashed=1;dashed=1;dashed=1;strokeColor=#B85450;" edge="1" parent="1" source="node7" target="node8">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="node8" value="结论：多倍体淀粉酶表达酵母MNIV/6GS可在无预处理、无外加酶和无营养补充下直接从高产水稻高效产乙醇，工艺易放大，具低成本潜力" style="shape=roundedRect;whiteSpace=wrap;html=1;fillColor=#C5E0B4;strokeColor=#538135;fontSize=13;fontStyle=1;verticalAlign=middle;perimeter=rectanglePoints;" vertex="1" parent="1">
      <mxGeometry x="450" y="162" width="340" height="73" as="geometry" />
    </mxCell>
    <mxCell id="edge8" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0;exitY=0.5;exitDx=0;exitDy=0;entryX=0;entryY=0.5;entryDx=0;entryDy=0;dashed=1;dashed=1;dashed=1;strokeColor=#538135;dashed=1;" edge="1" parent="1" source="node7" target="node8">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge9" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0;exitY=0.5;exitDx=0;exitDy=0;entryX=0;entryY=0.5;entryDx=0;entryDy=0;dashed=1;dashed=1;dashed=1;strokeColor=#538135;dashed=1;" edge="1" parent="1" source="node3" target="node8">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge10" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#E67E22;dashed=1;" edge="1" parent="1" source="node1" target="node8">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
  </root>
</mxGraphModel></diagram></mxfile>