<mxfile host="app.diagrams.net"><diagram name="roadmap"><mxGraphModel dx="1422" dy="793" grid="1" gridSize="10" guides="1" tooltips="1" connect="1" arrows="1" fold="1" page="1" pageScale="1" pageWidth="1169" pageHeight="826" math="0" shadow="0">
  <root>
    <mxCell id="0" />
    <mxCell id="1" parent="0" />
    <mxCell id="n1" value="研究问题：生淀粉直接发酵产乙醇，降低成本" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#dae8fc;strokeColor=#6c8ebf;fontSize=12;fontStyle=1;absoluteArcSize=4;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="57" y="40" width="306" height="64" as="geometry" />
    </mxCell>
    <mxCell id="n2" value="策略：表面共展示α-淀粉酶与葡糖淀粉酶" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#d5e8d4;strokeColor=#82b366;fontSize=12;fontStyle=1;absoluteArcSize=4;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="68" y="162" width="285" height="64" as="geometry" />
    </mxCell>
    <mxCell id="n3" value="关键改造1：α-淀粉酶N端添加底物结合域(SBAI)" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#ffe6cc;strokeColor=#d79b00;fontSize=12;fontStyle=1;absoluteArcSize=4;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="46" y="284" width="327" height="64" as="geometry" />
    </mxCell>
    <mxCell id="n4" value="关键改造2：构建二倍体菌株MNII/6GSBAIAG" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#ffe6cc;strokeColor=#d79b00;fontSize=12;fontStyle=1;absoluteArcSize=4;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="64" y="406" width="292" height="64" as="geometry" />
    </mxCell>
    <mxCell id="n5" value="关键改造3：导入麦芽糖转运蛋白AGT1" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#ffe6cc;strokeColor=#d79b00;fontSize=12;fontStyle=1;absoluteArcSize=4;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="82" y="528" width="257" height="64" as="geometry" />
    </mxCell>
    <mxCell id="n6" value="结果：乙醇88.9 g/l，理论产率88.0%，酶活2486 U/l" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#f8cecc;strokeColor=#b85450;fontSize=12;fontStyle=1;absoluteArcSize=4;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="40" y="650" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="n7" value="重复发酵验证：23次循环酶活不降，产率稳定" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#f8cecc;strokeColor=#b85450;fontSize=12;fontStyle=1;absoluteArcSize=4;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="57" y="772" width="306" height="64" as="geometry" />
    </mxCell>
    <mxCell id="n8" value="结论：重组酵母具备工业应用潜力，降低成本" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#e1d5e7;strokeColor=#9673a6;fontSize=12;fontStyle=1;absoluteArcSize=4;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="57" y="894" width="306" height="64" as="geometry" />
    </mxCell>
    <mxCell id="e1" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;" edge="1" parent="1" source="n1" target="n2">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="e2" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;" edge="1" parent="1" source="n2" target="n3">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="e3" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;" edge="1" parent="1" source="n3" target="n4">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="e4" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;" edge="1" parent="1" source="n4" target="n5">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="e5" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;" edge="1" parent="1" source="n5" target="n6">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="e6" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;" edge="1" parent="1" source="n6" target="n7">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="e7" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;" edge="1" parent="1" source="n7" target="n8">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
  </root>
</mxGraphModel></diagram></mxfile>