<mxfile host="app.diagrams.net"><diagram name="roadmap"><mxGraphModel dx="1200" dy="800" grid="1" gridSize="10" guides="1" tooltips="1" connect="1" arrows="1" fold="1" page="1" pageScale="1" pageWidth="1400" pageHeight="900" math="0" shadow="0">
  <root>
    <mxCell id="0" />
    <mxCell id="1" parent="0" />

    
    <mxCell id="node1" vertex="1" parent="1" value="研究问题：酵母无法高效分泌异源纤维素酶" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#DAE8FC;strokeColor=#6C8EBF;fontSize=13;fontStyle=1;align=center;verticalAlign=middle;">
      <mxGeometry x="485" y="40" width="292" height="64" as="geometry" />
    </mxCell>

    
    <mxCell id="node2" vertex="1" parent="1" value="策略：全基因组缺失株文库筛选增强基因" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#D5E8D4;strokeColor=#82B366;fontSize=13;fontStyle=1;align=center;verticalAlign=middle;">
      <mxGeometry x="492" y="162" width="278" height="64" as="geometry" />
    </mxCell>

    
    <mxCell id="node3" vertex="1" parent="1" value="改造①：转化Ctcel8A至5197株缺失株" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#FFF2CC;strokeColor=#D6B656;fontSize=12;align=center;verticalAlign=middle;">
      <mxGeometry x="40" y="284" width="257" height="64" as="geometry" />
    </mxCell>

    
    <mxCell id="node4" vertex="1" parent="1" value="改造②：CMC水解圈高通量筛选" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#FFF2CC;strokeColor=#D6B656;fontSize=12;align=center;verticalAlign=middle;">
      <mxGeometry x="367" y="284" width="215" height="64" as="geometry" />
    </mxCell>

    
    <mxCell id="node5" vertex="1" parent="1" value="改造③：染色体整合验证排除质粒效应" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#FFF2CC;strokeColor=#D6B656;fontSize=12;align=center;verticalAlign=middle;">
      <mxGeometry x="652" y="284" width="264" height="64" as="geometry" />
    </mxCell>

    
    <mxCell id="node6" vertex="1" parent="1" value="改造④：构建多重基因组合缺失株" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#FFF2CC;strokeColor=#D6B656;fontSize=12;align=center;verticalAlign=middle;">
      <mxGeometry x="986" y="284" width="236" height="64" as="geometry" />
    </mxCell>

    
    <mxCell id="node7" vertex="1" parent="1" value="结果：鉴定55个增强缺失株，VPS3/VPS16效果最佳" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#F8CECC;strokeColor=#B85450;fontSize=13;fontStyle=1;align=center;verticalAlign=middle;">
      <mxGeometry x="464" y="406" width="334" height="64" as="geometry" />
    </mxCell>

    
    <mxCell id="node8" vertex="1" parent="1" value="结论：VPS3为关键靶点，rav1Δvps3Δ双缺失活性达3.7倍，为CBP乙醇生产提供新策略" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#E1D5E7;strokeColor=#9673A6;fontSize=13;fontStyle=1;align=center;verticalAlign=middle;">
      <mxGeometry x="461" y="528" width="340" height="64" as="geometry" />
    </mxCell>

    
    <mxCell id="edge1" edge="1" parent="1" source="node1" target="node2" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#6C8EBF;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>

    
    <mxCell id="edge2" edge="1" parent="1" source="node2" target="node3" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#82B366;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>

    
    <mxCell id="edge3" edge="1" parent="1" source="node2" target="node4" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#82B366;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>

    
    <mxCell id="edge4" edge="1" parent="1" source="node2" target="node5" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#82B366;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>

    
    <mxCell id="edge5" edge="1" parent="1" source="node2" target="node6" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#82B366;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>

    
    <mxCell id="edge6" edge="1" parent="1" source="node3" target="node7" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#D6B656;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>

    
    <mxCell id="edge7" edge="1" parent="1" source="node4" target="node7" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#D6B656;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>

    
    <mxCell id="edge8" edge="1" parent="1" source="node5" target="node7" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#D6B656;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>

    
    <mxCell id="edge9" edge="1" parent="1" source="node6" target="node7" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#D6B656;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>

    
    <mxCell id="edge10" edge="1" parent="1" source="node7" target="node8" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#B85450;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>

  </root>
</mxGraphModel></diagram></mxfile>