<mxfile host="app.diagrams.net"><diagram name="roadmap"><mxGraphModel dx="1200" dy="800" grid="1" gridSize="10" guides="1" tooltips="1" connect="1" arrows="1" fold="1" page="1" pageScale="1" pageWidth="1400" pageHeight="900" math="0" shadow="0">
  <root>
    <mxCell id="0" />
    <mxCell id="1" parent="0" />
    
    
    <mxCell id="node1" value="研究问题：异源Atlcb基因表达能否诱导亚麻内源Lulcb基因共抑制及其机制" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#dae8fc;strokeColor=#6c8ebf;fontSize=13;fontStyle=1;verticalAlign=middle;arcSize=8;" vertex="1" parent="1">
      <mxGeometry x="450" y="40" width="340" height="64" as="geometry" />
    </mxCell>
    
    
    <mxCell id="node2" value="策略A：构建转基因亚麻株系L9和L18（异源表达Atlcb基因）" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#fff2cc;strokeColor=#d6b656;fontSize=12;verticalAlign=middle;arcSize=8;" vertex="1" parent="1">
      <mxGeometry x="245" y="162" width="340" height="64" as="geometry" />
    </mxCell>
    
    
    <mxCell id="node3" value="策略B：检测内源Lulcb表达及表型变化（基因沉默、类胡萝卜素含量）" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#fff2cc;strokeColor=#d6b656;fontSize=12;verticalAlign=middle;arcSize=8;" vertex="1" parent="1">
      <mxGeometry x="655" y="162" width="340" height="64" as="geometry" />
    </mxCell>
    
    
    <mxCell id="node4" value="关键改造①：甲基化敏感酶切分析内源Lulcb基因甲基化模式+基因组总甲基化水平+DNA甲基化相关基因表达检测" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#f8cecc;strokeColor=#b85450;fontSize=12;verticalAlign=middle;arcSize=8;" vertex="1" parent="1">
      <mxGeometry x="40" y="284" width="340" height="64" as="geometry" />
    </mxCell>
    
    
    <mxCell id="node5" value="关键改造②：氮杂胞苷（AzaC）处理验证甲基化对Lulcb基因表达的调控作用" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#f8cecc;strokeColor=#b85450;fontSize=12;verticalAlign=middle;arcSize=8;" vertex="1" parent="1">
      <mxGeometry x="450" y="284" width="340" height="64" as="geometry" />
    </mxCell>
    
    
    <mxCell id="node6" value="关键改造③：分析类胡萝卜素代谢、ABA合成/分解基因表达，并外源ABA处理验证几丁质酶调控" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#f8cecc;strokeColor=#b85450;fontSize=12;verticalAlign=middle;arcSize=8;" vertex="1" parent="1">
      <mxGeometry x="860" y="284" width="340" height="64" as="geometry" />
    </mxCell>
    
    
    <mxCell id="node7" value="结果：①Lulcb表达下调50%，基因体+297位点甲基化增加12%-22%；总甲基化降低12%-23%；②AzaC处理后Lulcb转录恢复至92%-89%；③ABA含量升高62%-27%，CYP707A下降&gt;99%，几丁质酶升高11-9倍" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#d5e8d4;strokeColor=#82b366;fontSize=12;verticalAlign=middle;arcSize=8;" vertex="1" parent="1">
      <mxGeometry x="450" y="406" width="340" height="90" as="geometry" />
    </mxCell>
    
    
    <mxCell id="node8" value="结论：Atlcb异源表达引发内源Lulcb基因体甲基化重排（CMT3↑，ROS1/DME↓）致其沉默，减少类胡萝卜素合成但抑制ABA分解升高ABA含量，激活几丁质酶基因增强镰刀菌抗性" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#e1d5e7;strokeColor=#9673a6;fontSize=13;fontStyle=1;verticalAlign=middle;arcSize=8;" vertex="1" parent="1">
      <mxGeometry x="450" y="554" width="340" height="73" as="geometry" />
    </mxCell>
    
    
    <mxCell id="edge1" edge="1" parent="1" source="node1" target="node2" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#6c8ebf;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    
    
    <mxCell id="edge2" edge="1" parent="1" source="node1" target="node3" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#6c8ebf;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    
    
    <mxCell id="edge3" edge="1" parent="1" source="node2" target="node4" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#b85450;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    
    
    <mxCell id="edge4" edge="1" parent="1" source="node2" target="node5" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#b85450;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    
    
    <mxCell id="edge5" edge="1" parent="1" source="node3" target="node6" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#b85450;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    
    
    <mxCell id="edge6" edge="1" parent="1" source="node4" target="node7" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#82b366;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    
    
    <mxCell id="edge7" edge="1" parent="1" source="node5" target="node7" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#82b366;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    
    
    <mxCell id="edge8" edge="1" parent="1" source="node6" target="node7" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#82b366;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    
    
    <mxCell id="edge9" edge="1" parent="1" source="node7" target="node8" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#9673a6;">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    
  </root>
</mxGraphModel></diagram></mxfile>