<mxfile host="app.diagrams.net"><diagram name="roadmap"><mxGraphModel dx="1200" dy="800" grid="1" gridSize="10" guides="1" tooltips="1" connect="1" arrows="1" fold="1" page="1" pageScale="1" pageWidth="1400" pageHeight="900" math="0" shadow="0">
  <root>
    <mxCell id="0" />
    <mxCell id="1" parent="0" />

    <!-- 节点1：研究问题 -->
    <mxCell id="node1" value="莽草酸传统提取成本高，需开发发酵法" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#FFE6CC;strokeColor=#D79B00;fontSize=13;fontStyle=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="50" y="80" width="220" height="60" as="geometry" />
    </mxCell>

    <!-- 节点2：整体策略 -->
    <mxCell id="node2" value="代谢工程改造大肠杆菌/枯草芽孢杆菌/柠檬酸杆菌" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#D5E8D4;strokeColor=#82B366;fontSize=13;fontStyle=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="370" y="80" width="240" height="60" as="geometry" />
    </mxCell>

    <!-- 节点3：阻断下游代谢 -->
    <mxCell id="node3" value="阻断莽草酸下游代谢（敲除aroK/aroL或aroA）" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#DAE3F3;strokeColor=#6C8EBF;fontSize=13;fontStyle=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <x="370" y="80" as="geometry" />
      <mxGeometry x="50" y="220" width="240" height="60" as="geometry" />
    </mxCell>

    <!-- 节点4：强化前体供给 -->
    <mxCell id="node4" value="强化中心碳代谢，增加PEP和E4P供给" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#DAE3F3;strokeColor=#6C8EBF;fontSize=13;fontStyle=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="370" y="220" width="240" height="60" as="geometry" />
    </mxCell>

    <!-- 节点5：关键改造细节 -->
    <mxCell id="node5" value="关键改造：PTS替换(glf/glk)、过表达tktA/ppsA、底物通道aroD·E、敲除shiA" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#F8CECC;strokeColor=#B85450;fontSize=13;fontStyle=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="720" y="80" width="260" height="80" as="geometry" />
    </mxCell>

    <!-- 节点6：关键结果1（大肠杆菌最佳菌株） -->
    <mxCell id="node6" value="大肠杆菌最佳菌株：10-L发酵罐84 g/L，产率0.33 mol/mol" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#FFF2CC;strokeColor=#D6B656;fontSize=13;fontStyle=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="50" y="380" width="260" height="60" as="geometry" />
    </mxCell>

    <!-- 节点7：关键结果2（前体强化效果） -->
    <mxCell id="node7" value="过表达tktA：38→52 g/L；过表达ppsA：66 g/L" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#FFF2CC;strokeColor=#D6B656;fontSize=13;fontStyle=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="370" y="380" width="240" height="60" as="geometry" />
    </mxCell>

    <!-- 节点8：关键结果3（其他菌株） -->
    <mxCell id="node8" value="枯草芽孢杆菌19.7 g/L；EPSP缺陷大肠杆菌20 g/L" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#FFF2CC;strokeColor=#D6B656;fontSize=13;fontStyle=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="720" y="380" width="260" height="60" as="geometry" />
    </mxCell>

    <!-- 节点9：结论 -->
    <mxCell id="node9" value="结论：代谢工程实现莽草酸高效发酵生产，为达菲提供可持续原料" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#E1D5E7;strokeColor=#9673A6;fontSize=14;fontStyle=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="370" y="560" width="300" height="60" as="geometry" />
    </mxCell>

    <!-- 连线1：节点1 → 节点2 -->
    <mxCell id="edge1" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;fontSize=11;" edge="1" parent="1" source="node1" target="node2">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>

    <!-- 连线2：节点2 → 节点3 -->
    <mxCell id="edge2" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;fontSize=11;" edge="1" parent="1" source="node2" target="node3">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>

    <!-- 连线3：节点2 → 节点4 -->
    <mxCell id="edge3" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;fontSize=11;" edge="1" parent="1" source="node2" target="node4">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>

    <!-- 连线4：节点3 → 节点5 -->
    <mxCell id="edge4" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;fontSize=11;" edge="1" parent="1" source="node3" target="node5">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>

    <!-- 连线5：节点4 → 节点5 -->
    <mxCell id="edge5" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;fontSize=11;" edge="1" parent="1" source="node4" target="node5">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>

    <!-- 连线6：节点5 → 节点6 -->
    <mxCell id="edge6" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;fontSize=11;" edge="1" parent="1" source="node5" target="node6">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>

    <!-- 连线7：节点5 → 节点7 -->
    <mxCell id="edge7" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;fontSize=11;" edge="1" parent="1" source="node5" target="node7">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>

    <!-- 连线8：节点5 → 节点8 -->
    <mxCell id="edge8" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;fontSize=11;" edge="1" parent="1" source="node5" target="node8">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>

    <!-- 连线9：节点6 → 节点9 -->
    <mxCell id="edge9" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;fontSize=11;" edge="1" parent="1" source="node6" target="node9">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>

    <!-- 连线10：节点7 → 节点9 -->
    <mxCell id="edge10" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;fontSize=11;" edge="1" parent="1" source="node7" target="node9">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>

    <!-- 连线11：节点8 → 节点9 -->
    <mxCell id="edge11" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;fontSize=11;" edge="1" parent="1" source="node8" target="node9">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>

  </root>
</mxGraphModel></diagram></mxfile>