<mxfile host="app.diagrams.net"><diagram name="roadmap"><mxGraphModel dx="1200" dy="800" grid="1" gridSize="10" guides="1" tooltips="1" connect="1" arrows="1" fold="1" page="1" pageScale="1" pageWidth="1200" pageHeight="800" math="0" shadow="0">
  <root>
    <mxCell id="0" />
    <mxCell id="1" parent="0" />
    <mxCell id="node1" value="研究问题：异源宿主高效合成香叶醇，解析VoGES/LdGES定位差异" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#D9EAD3;strokeColor=#6AA84F;fontSize=12;fontStyle=1;verticalAlign=middle;align=center;absoluteArcSize=8;" vertex="1" parent="1">
      <mxGeometry x="812" y="40" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node2" value="策略：克隆表达VoGES/LdGES，体外酶学+体内定位与代谢分析" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#FFF2CC;strokeColor=#BF9000;fontSize=12;fontStyle=1;verticalAlign=middle;align=center;absoluteArcSize=8;" vertex="1" parent="1">
      <mxGeometry x="812" y="162" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node3" value="关键改造：GFP融合定位、截短突变（ΔN）、人工质体转运肽（pΔN）" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#CFE2F3;strokeColor=#3D85C6;fontSize=12;fontStyle=1;verticalAlign=middle;align=center;absoluteArcSize=8;" vertex="1" parent="1">
      <mxGeometry x="40" y="284" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node4" value="结果：体外Km相似（32 vs 51 μM），GPP专一" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#F4CCCC;strokeColor=#CC0000;fontSize=12;fontStyle=1;verticalAlign=middle;align=center;absoluteArcSize=8;" vertex="1" parent="1">
      <mxGeometry x="450" y="284" width="313" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node5" value="结果：定位不同但体内活性相似（质体 vs 胞质）" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#F4CCCC;strokeColor=#CC0000;fontSize=12;fontStyle=1;verticalAlign=middle;align=center;absoluteArcSize=8;" vertex="1" parent="1">
      <mxGeometry x="833" y="284" width="334" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node6" value="结果：胞质缩短型产量仅低30%，GPP可及" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#F4CCCC;strokeColor=#CC0000;fontSize=12;fontStyle=1;verticalAlign=middle;align=center;absoluteArcSize=8;" vertex="1" parent="1">
      <mxGeometry x="1237" y="284" width="278" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node7" value="结果：19种糖基化产物，氧化/糖基化代谢分流显著" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#F4CCCC;strokeColor=#CC0000;fontSize=12;fontStyle=1;verticalAlign=middle;align=center;absoluteArcSize=8;" vertex="1" parent="1">
      <mxGeometry x="1585" y="284" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node8" value="结果：瞬时与稳定表达产量差异（0.58 vs 0.05 μg/g fw/h）" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#F4CCCC;strokeColor=#CC0000;fontSize=12;fontStyle=1;verticalAlign=middle;align=center;absoluteArcSize=8;" vertex="1" parent="1">
      <mxGeometry x="608" y="406" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="node9" value="结论：胞质GPP足以供单萜合成；重建TIA途径需控分流" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#D9EAD3;strokeColor=#6AA84F;fontSize=12;fontStyle=1;verticalAlign=middle;align=center;absoluteArcSize=8;" vertex="1" parent="1">
      <mxGeometry x="1018" y="406" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="edge1" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#6AA84F;" edge="1" parent="1" source="node1" target="node2">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge2" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#BF9000;" edge="1" parent="1" source="node2" target="node3">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge3" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#BF9000;" edge="1" parent="1" source="node2" target="node4">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge4" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#BF9000;" edge="1" parent="1" source="node2" target="node5">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge5" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#BF9000;" edge="1" parent="1" source="node2" target="node6">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge6" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#BF9000;" edge="1" parent="1" source="node2" target="node7">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge7" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=1;exitY=0.5;exitDx=0;exitDy=0;entryX=0;entryY=0.5;entryDx=0;entryDy=0;strokeColor=#CC0000;" edge="1" parent="1" source="node4" target="node5">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge8" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=1;exitY=0.5;exitDx=0;exitDy=0;entryX=0;entryY=0.5;entryDx=0;entryDy=0;strokeColor=#CC0000;" edge="1" parent="1" source="node5" target="node6">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge9" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=1;exitY=0.5;exitDx=0;exitDy=0;entryX=0;entryY=0.5;entryDx=0;entryDy=0;strokeColor=#CC0000;" edge="1" parent="1" source="node6" target="node7">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge10" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#CC0000;" edge="1" parent="1" source="node4" target="node8">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge11" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=1;exitY=0.5;exitDx=0;exitDy=0;entryX=0;entryY=0.5;entryDx=0;entryDy=0;strokeColor=#CC0000;" edge="1" parent="1" source="node8" target="node9">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="edge12" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#6AA84F;dashed=1;" edge="1" parent="1" source="node7" target="node9">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
  </root>
</mxGraphModel></diagram></mxfile>