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    <mxCell id="node2" value="理性设计：构建荧光报告基团库表征启动子/RBS/降解标签（&gt;5个数量级），组合多层级调控（基因剂量/转录后/翻译后）抑制低锌crtY表达，并引入pJBEI-MEV途径补充前体" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#FFD966;strokeColor=#B08F20;verticalAlign=middle;align=center;fontSize=12;fontColor=#000000;" vertex="1" parent="1">
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    <mxCell id="node3" value="关键改造：1. 改用低拷贝质粒pSB6A1 2. 组合中等RBS（B0033）和LAA降解标签 3. 整合violacein通路（PznuC控制vioABCDE） 4. 共转化pJBEI-MEV质粒" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#A6D9A8;strokeColor=#4F9A54;verticalAlign=middle;align=center;fontSize=12;fontColor=#000000;" vertex="1" parent="1">
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    <mxCell id="node4" value="结果1：荧光库显示表达调控范围超过5个数量级，PznTA基线表达高于J23117，LAA标签降蛋白3个数量级" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#C5B9D1;strokeColor=#7E6B9E;verticalAlign=middle;align=center;fontSize=11;fontColor=#000000;" vertex="1" parent="1">
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    <mxCell id="node5" value="结果2：仅换RBS（B0033）不足以产生番茄红素态；出现启动子突变和双峰群体（B0034高表达压力）" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#C5B9D1;strokeColor=#7E6B9E;verticalAlign=middle;align=center;fontSize=11;fontColor=#000000;" vertex="1" parent="1">
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    <mxCell id="node6" value="结果3：低拷贝质粒+RBS+LAA后获得红/橙双离散状态；pJBEI-MEV共转化使番茄红素产量提高3倍，切换点移至50 μM Zn²⁺" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#C5B9D1;strokeColor=#7E6B9E;verticalAlign=middle;align=center;fontSize=11;fontColor=#000000;" vertex="1" parent="1">
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    <mxCell id="node7" value="结果：初始菌株仅紫/橙两色，改造后获得红/橙离散状态；前体补充可调节颜色切换锌浓度" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#D9A6A6;strokeColor=#A94F4F;verticalAlign=middle;align=center;fontSize=12;fontColor=#000000;" vertex="1" parent="1">
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    <mxCell id="node8" value="结论：多层级组合调控精确控制类胡萝卜素通路状态，实现色素输出生物传感器；该精密代谢工程策略可推广至其他受限天然感应系统和多功能细胞工厂开发" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#99B7AD;strokeColor=#3F6E63;verticalAlign=middle;align=center;fontSize=12;fontColor=#000000;" vertex="1" parent="1">
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