<mxfile host="app.diagrams.net"><diagram name="roadmap"><mxGraphModel dx="1422" dy="762" grid="1" gridSize="10" guides="1" tooltips="1" connect="1" arrows="1" fold="1" page="1" pageScale="1" pageWidth="1400" pageHeight="900" math="0" shadow="0">
  <root>
    <mxCell id="0" />
    <mxCell id="1" parent="0" />

    
    <mxCell id="problem" value="核心问题：核基因组多基因工程面临基因沉默、位置效应、耗时杂交及基因扩散等瓶颈" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#DEE8F5;strokeColor=#5B9BD5;fontSize=13;fontStyle=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="245" y="40" width="340" height="64" as="geometry" />
    </mxCell>

    
    <mxCell id="strategy" value="研究策略：系统比较核/叶绿体多基因表达，聚焦叶绿体多顺反子及其协同调控策略" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#E2F0D9;strokeColor=#70AD47;fontSize=13;fontStyle=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="245" y="162" width="340" height="64" as="geometry" />
    </mxCell>

    
    <mxCell id="innovation1" value="关键改造①：多顺反子表达与操纵子导入（HSA、Cry、PHB等）" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#FFF2CC;strokeColor=#FFC000;fontSize=13;fontStyle=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="245" y="284" width="340" height="64" as="geometry" />
    </mxCell>

    
    <mxCell id="innovation2" value="关键改造②：植物源BADH标记取代抗生素筛选" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#FFF2CC;strokeColor=#FFC000;fontSize=13;fontStyle=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="57" y="406" width="306" height="64" as="geometry" />
    </mxCell>

    
    <mxCell id="innovation3" value="关键改造③：异源UTR/5’序列增强易降解蛋白表达" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#FFF2CC;strokeColor=#FFC000;fontSize=13;fontStyle=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="40" y="528" width="340" height="64" as="geometry" />
    </mxCell>

    
    <mxCell id="innovation4" value="关键改造④：定点同源重组与母系遗传（避免位置效应/基因扩散）" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#FFF2CC;strokeColor=#FFC000;fontSize=13;fontStyle=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="40" y="650" width="340" height="64" as="geometry" />
    </mxCell>

    
    <mxCell id="result1" value="结果①：金稻β-胡萝卜素；拟南芥PHB达4%鲜重；41倍霍乱毒素B表达" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#FDE9E9;strokeColor=#E74C3C;fontSize=13;fontStyle=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="433" y="406" width="340" height="64" as="geometry" />
    </mxCell>

    
    <mxCell id="result2" value="结果②：Cry2Aa2操纵子达46.1% tsp；抗菌肽/海藻糖/抗体等高效积累" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#FDE9E9;strokeColor=#E74C3C;fontSize=13;fontStyle=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="450" y="650" width="340" height="64" as="geometry" />
    </mxCell>

    
    <mxCell id="result3" value="结果③：无多效性/无位置效应/免抗生素再生（BADH两周内）" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#FDE9E9;strokeColor=#E74C3C;fontSize=13;fontStyle=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="245" y="772" width="340" height="64" as="geometry" />
    </mxCell>

    
    <mxCell id="conclusion" value="结论：叶绿体基因工程＝后基因组时代多基因表达/代谢工程核心平台（单启动子多顺反子+无沉默+基因限制）" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#E1D5E7;strokeColor=#9C60B0;fontSize=14;fontStyle=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="450" y="528" width="340" height="64" as="geometry" />
    </mxCell>

    
    <mxCell id="e1" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#5B9BD5;" edge="1" parent="1" source="problem" target="strategy">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>

    
    <mxCell id="e2" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#FFC000;" edge="1" parent="1" source="strategy" target="innovation1">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>

    
    <mxCell id="e3" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#FFC000;" edge="1" parent="1" source="innovation1" target="innovation2">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>

    
    <mxCell id="e4" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#FFC000;" edge="1" parent="1" source="innovation2" target="innovation3">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>

    
    <mxCell id="e5" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#FFC000;" edge="1" parent="1" source="innovation3" target="innovation4">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>

    
    <mxCell id="e6" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#E74C3C;" edge="1" parent="1" source="innovation1" target="result1">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>

    
    <mxCell id="e7" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#E74C3C;" edge="1" parent="1" source="innovation3" target="result2">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>

    
    <mxCell id="e8" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#E74C3C;" edge="1" parent="1" source="innovation4" target="result3">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>

    
    <mxCell id="e9" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#9C60B0;" edge="1" parent="1" source="result1" target="conclusion">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>

    
    <mxCell id="e10" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0;exitY=0.5;exitDx=0;exitDy=0;entryX=0;entryY=0.5;entryDx=0;entryDy=0;dashed=1;dashed=1;strokeColor=#9C60B0;" edge="1" parent="1" source="result2" target="conclusion">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>

    
    <mxCell id="e11" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0;exitY=0.5;exitDx=0;exitDy=0;entryX=0;entryY=0.5;entryDx=0;entryDy=0;dashed=1;dashed=1;strokeColor=#9C60B0;" edge="1" parent="1" source="result3" target="conclusion">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>

  </root>
</mxGraphModel></diagram></mxfile>