<mxfile host="app.diagrams.net"><diagram name="roadmap"><mxGraphModel dx="1422" dy="793" grid="1" gridSize="10" guides="1" tooltips="1" connect="1" arrows="1" fold="1" page="1" pageScale="1" pageWidth="1169" pageHeight="826" math="0" shadow="0">
  <root>
    <mxCell id="0" />
    <mxCell id="1" parent="0" />
    
    
    <mxCell id="node1" value="P450cam选择性氧化(+)-α-pinene生成高值萜类衍生物" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#dae8fc;strokeColor=#6c8ebf;fontSize=13;fontStyle=1;width=180;height=70;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="398" y="40" width="340" height="64" as="geometry" />
    </mxCell>
    
    
    <mxCell id="node2" value="结构对比解析设计P450cam活性位点突变" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#d5e8d4;strokeColor=#82b366;fontSize=13;fontStyle=1;width=180;height=70;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="432" y="162" width="271" height="64" as="geometry" />
    </mxCell>
    
    
    <mxCell id="node3" value="构建Y96F、F87A/L/W、V247L、L244A突变体库" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#fff2cc;strokeColor=#d6b656;fontSize=13;width=180;height=70;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="244" y="284" width="306" height="64" as="geometry" />
    </mxCell>
    
    
    <mxCell id="node4" value="解析F87W/Y96F/V247L复合物晶体结构（2.3 Å）" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#fff2cc;strokeColor=#d6b656;fontSize=13;width=180;height=70;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="202" y="406" width="327" height="73" as="geometry" />
    </mxCell>
    
    
    <mxCell id="node5" value="基于接触分析迭代突变优化选择性" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#fff2cc;strokeColor=#d6b656;fontSize=13;width=180;height=70;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="40" y="537" width="236" height="64" as="geometry" />
    </mxCell>
    
    
    <mxCell id="node6" value="筛选出高活性、高选择性P450cam突变体" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#ffe6cc;strokeColor=#d79b00;fontSize=13;fontStyle=1;width=220;height=70;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="620" y="284" width="271" height="64" as="geometry" />
    </mxCell>
    
    
    <mxCell id="node7" value="△ 选择性：F87W/Y96F/L244A产86% cis-verbenol&#10;△ 活性：Y96F/V247L达270 nmol/min/nmol&#10;△ 偶联效率：&gt;95%，区域选择性91%" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#ffe6cc;strokeColor=#d79b00;fontSize=12;width=240;height=80;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="600" y="406" width="334" height="73" as="geometry" />
    </mxCell>
    
    
    <mxCell id="node8" value="底物双取向结合：C4距铁3.40 Å → cis-verbenol，C10距铁2.70 Å → myrtenol" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#e1d5e7;strokeColor=#9673a6;fontSize=12;width=240;height=70;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="346" y="537" width="340" height="64" as="geometry" />
    </mxCell>
    
    
    <mxCell id="node9" value="结论：理性设计+结构指导实现非天然底物高效选择性氧化，Y96F/L244A/V247L可实现α-pinene直接氧化生成verbenone" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#f8cecc;strokeColor=#b85450;fontSize=13;fontStyle=1;width=260;height=80;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="756" y="537" width="340" height="64" as="geometry" />
    </mxCell>
    
    
    <mxCell id="edge1" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#6c8ebf;" edge="1" parent="1" source="node1" target="node2">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    
    
    <mxCell id="edge2" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#82b366;" edge="1" parent="1" source="node2" target="node3">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    
    
    <mxCell id="edge3" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#d6b656;" edge="1" parent="1" source="node3" target="node4">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    
    
    <mxCell id="edge4" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#d6b656;" edge="1" parent="1" source="node4" target="node5">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    
    
    <mxCell id="edge5" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#82b366;dashed=1;" edge="1" parent="1" source="node2" target="node6">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    
    
    <mxCell id="edge6" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0;exitY=0.5;exitDx=0;exitDy=0;entryX=0;entryY=0.5;entryDx=0;entryDy=0;dashed=1;dashed=1;dashed=1;dashed=1;dashed=1;strokeColor=#d6b656;" edge="1" parent="1" source="node5" target="node6">
      <mxGeometry relative="1" as="geometry">
        </mxGeometry>
    </mxCell>
    
    
    <mxCell id="edge7" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#d79b00;" edge="1" parent="1" source="node6" target="node7">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    
    
    <mxCell id="edge8" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#9673a6;dashed=1;" edge="1" parent="1" source="node4" target="node8">
      <mxGeometry relative="1" as="geometry">
        </mxGeometry>
    </mxCell>
    
    
    <mxCell id="edge9" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#b85450;" edge="1" parent="1" source="node7" target="node9">
      <mxGeometry relative="1" as="geometry">
        </mxGeometry>
    </mxCell>
    
    
    <mxCell id="edge10" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=1;exitY=0.5;exitDx=0;exitDy=0;entryX=0;entryY=0.5;entryDx=0;entryDy=0;strokeColor=#9673a6;dashed=1;" edge="1" parent="1" source="node8" target="node9">
      <mxGeometry relative="1" as="geometry">
        </mxGeometry>
    </mxCell>
    
  </root>
</mxGraphModel></diagram></mxfile>