<mxfile host="app.diagrams.net"><diagram name="roadmap"><mxGraphModel dx="1200" dy="800" grid="1" gridSize="10" guides="1" tooltips="1" connect="1" arrows="1" fold="1" page="1" pageScale="1" pageWidth="1400" pageHeight="900" math="0" shadow="0">
  <root>
    <mxCell id="0" />
    <mxCell id="1" parent="0" />
    
    
    <mxCell id="n1" value="核心问题：现有遗传工程方法无法并行、连续地定向进化基因组，难以产生新表型" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#FFF2CC;strokeColor=#D6B656;fontSize=12;fontStyle=0;verticalAlign=middle;align=center;arcSize=8;fontColor=#333333;" vertex="1" parent="1">
      <mxGeometry x="40" y="40" width="340" height="64" as="geometry" />
    </mxCell>
    
    
    <mxCell id="n2" value="策略开发：设计MAGE—多重自动化基因组工程+加速进化策略" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#E1D5E7;strokeColor=#9673A6;fontSize=12;fontStyle=0;verticalAlign=middle;align=center;arcSize=8;fontColor=#333333;" vertex="1" parent="1">
      <mxGeometry x="40" y="214" width="340" height="64" as="geometry" />
    </mxCell>
    
    
    <mxCell id="n3" value="关键技术：λ-Red重组系统+单链DNA寡核苷酸池，靶向24个染色体位点并行修饰" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#D9E1F2;strokeColor=#8FAADC;fontSize=12;fontStyle=0;verticalAlign=middle;align=center;arcSize=8;fontColor=#333333;" vertex="1" parent="1">
      <mxGeometry x="40" y="388" width="340" height="64" as="geometry" />
    </mxCell>
    
    
    <mxCell id="n4" value="自动化设备驱动连续循环：每周期2~2.5h，等位替换效率&gt;30%，日变异4.3×10⁹" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#D9E1F2;strokeColor=#8FAADC;fontSize=12;fontStyle=0;verticalAlign=middle;align=center;arcSize=8;fontColor=#333333;" vertex="1" parent="1">
      <mxGeometry x="40" y="562" width="340" height="64" as="geometry" />
    </mxCell>
    
    
    <mxCell id="n5" value="基因组多样性实现：cN6池5轮洗涤后每细胞3.1个碱基变化，15轮覆盖全部4096种N6组合" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#C6EFCE;strokeColor=#70AD47;fontSize=12;fontStyle=0;verticalAlign=middle;align=center;arcSize=8;fontColor=#333333;" vertex="1" parent="1">
      <mxGeometry x="40" y="736" width="340" height="64" as="geometry" />
    </mxCell>
    
    
    <mxCell id="n6" value="途径优化验证：DXP途径24基因同步修饰，日变异150亿；筛选获番茄红素产量提升5倍（~9000 μg/g干重）" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#C6EFCE;strokeColor=#70AD47;fontSize=12;fontStyle=0;verticalAlign=middle;align=center;arcSize=8;fontColor=#333333;" vertex="1" parent="1">
      <mxGeometry x="40" y="910" width="340" height="64" as="geometry" />
    </mxCell>
    
    
    <mxCell id="n7" value="基因组合效应：激活idi提升40%；同步提升dxs+idi提升390%；敲除gdhA增产但减速32%" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#C6EFCE;strokeColor=#70AD47;fontSize=12;fontStyle=0;verticalAlign=middle;align=center;arcSize=8;fontColor=#333333;" vertex="1" parent="1">
      <mxGeometry x="40" y="1084" width="340" height="64" as="geometry" />
    </mxCell>
    
    
    <mxCell id="n8" value="结论：MAGE是一种高效、廉价、自动化的多重基因组工程技术，广泛应用于代谢工程与合成生物学" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#FCE4D6;strokeColor=#D08949;fontSize=12;fontStyle=0;verticalAlign=middle;align=center;arcSize=8;fontColor=#333333;" vertex="1" parent="1">
      <mxGeometry x="40" y="1258" width="340" height="64" as="geometry" />
    </mxCell>
    
    
    
    <mxCell id="e1" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#555555;" edge="1" parent="1" source="n1" target="n2">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    
    
    <mxCell id="e2" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#555555;" edge="1" parent="1" source="n2" target="n3">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    
    
    <mxCell id="e3" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#555555;" edge="1" parent="1" source="n3" target="n4">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    
    
    <mxCell id="e4" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#555555;" edge="1" parent="1" source="n4" target="n5">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    
    
    <mxCell id="e5" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#555555;" edge="1" parent="1" source="n5" target="n6">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    
    
    <mxCell id="e6" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#555555;" edge="1" parent="1" source="n6" target="n7">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    
    
    <mxCell id="e7" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#D08949;" edge="1" parent="1" source="n7" target="n8">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
  </root>
</mxGraphModel></diagram></mxfile>