<mxfile host="app.diagrams.net"><diagram name="roadmap"><mxGraphModel dx="1422" dy="794" grid="1" gridSize="10" guides="1" tooltips="1" connect="1" arrows="1" fold="1" page="1" pageScale="1" pageWidth="1600" pageHeight="900" math="0" shadow="0">
  <root>
    <mxCell id="0" />
    <mxCell id="1" parent="0" />

    
    <mxCell id="node1" value="问题：CO2排放与能源危机，需直接光合合成异丁醛替代化石燃料" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#dae8fc;strokeColor=#6c8ebf;fontSize=13;fontStyle=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="450" y="40" width="340" height="64" as="geometry" />
    </mxCell>

    
    <mxCell id="node2" value="策略：基因工程将异丁醛合成途径（kivd, alsS, ilvC, ilvD）整合到蓝藻基因组" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#d5e8d4;strokeColor=#82b366;fontSize=13;fontStyle=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="450" y="162" width="340" height="64" as="geometry" />
    </mxCell>

    
    <mxCell id="node3" value="改造1：过表达Rubisco（rbcLS）增强CO2固定" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#fff2cc;strokeColor=#d6b656;fontSize=13;fontStyle=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="262" y="284" width="306" height="64" as="geometry" />
    </mxCell>

    
    <mxCell id="node4" value="改造2：利用异丁醛高挥发性，气体汽提原位回收产物" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#fff2cc;strokeColor=#d6b656;fontSize=13;fontStyle=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="40" y="406" width="340" height="64" as="geometry" />
    </mxCell>

    
    <mxCell id="node5" value="结果1：表达kivd的SA590，12天产723 mg/L异丁醛，速率2500 μg/L/h" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#f8cecc;strokeColor=#b85450;fontSize=13;fontStyle=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="638" y="284" width="340" height="64" as="geometry" />
    </mxCell>

    
    <mxCell id="node6" value="结果2：过表达rbcLS的SA665，8天产1.1 g/L，速率6230 μg/L/h，Rubisco活性↑1.4倍" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#f8cecc;strokeColor=#b85450;fontSize=13;fontStyle=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="450" y="406" width="340" height="64" as="geometry" />
    </mxCell>

    
    <mxCell id="node7" value="结果3：SA579产450 mg/L异丁醇；异丁醛耐受浓度750 mg/L；生产率超乙醇/氢/油脂" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#f8cecc;strokeColor=#b85450;fontSize=13;fontStyle=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="450" y="528" width="340" height="64" as="geometry" />
    </mxCell>

    
    <mxCell id="node8" value="结论：直接光合转化CO2为燃料可行；过表达Rubisco与原位回收显著提高生产率，应对能源与气候变化" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#e1d5e7;strokeColor=#9673a6;fontSize=13;fontStyle=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
      <mxGeometry x="860" y="406" width="340" height="64" as="geometry" />
    </mxCell>

    
    <mxCell style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;" id="edge1" edge="1" parent="1" source="node1" target="node2">
      <mxGeometry relative="1" as="geometry" />
      <mxPoint x="340" y="90" as="sourcePoint" />
      <mxPoint x="410" y="90" as="targetPoint" />
    </mxCell>
    <mxCell id="edge1style" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#6c8ebf;" edge="1" parent="1" source="node1" target="node2">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>

    
    <mxCell style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;" id="edge2" edge="1" parent="1" source="node2" target="node3">
      <mxGeometry relative="1" as="geometry" />
      <mxPoint x="690" y="90" as="sourcePoint" />
      <mxPoint x="760" y="90" as="targetPoint" />
    </mxCell>
    <mxCell id="edge2style" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#82b366;" edge="1" parent="1" source="node2" target="node3">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>

    
    <mxCell style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;" id="edge3" edge="1" parent="1" source="node3" target="node4">
      <mxGeometry relative="1" as="geometry" />
      <mxPoint x="1040" y="90" as="sourcePoint" />
      <mxPoint x="1110" y="90" as="targetPoint" />
    </mxCell>
    <mxCell id="edge3style" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#d6b656;" edge="1" parent="1" source="node3" target="node4">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>

    
    <mxCell style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;" id="edge4" edge="1" parent="1" source="node2" target="node5">
      <mxGeometry relative="1" as="geometry" />
      <mxPoint x="550" y="220" as="sourcePoint" />
      <mxPoint x="550" y="260" as="targetPoint" />
    </mxCell>
    <mxCell id="edge4style" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#82b366;" edge="1" parent="1" source="node2" target="node5">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>

    
    <mxCell style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;" id="edge5" edge="1" parent="1" source="node3" target="node6">
      <mxGeometry relative="1" as="geometry" />
      <mxPoint x="900" y="220" as="sourcePoint" />
      <mxPoint x="900" y="260" as="targetPoint" />
    </mxCell>
    <mxCell id="edge5style" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#d6b656;" edge="1" parent="1" source="node3" target="node6">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>

    
    <mxCell style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;" id="edge6" edge="1" parent="1" source="node4" target="node7">
      <mxGeometry relative="1" as="geometry" />
      <mxPoint x="1250" y="220" as="sourcePoint" />
      <mxPoint x="1250" y="260" as="targetPoint" />
    </mxCell>
    <mxCell id="edge6style" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#d6b656;" edge="1" parent="1" source="node4" target="node7">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>

    
    <mxCell style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;" id="edge7" edge="1" parent="1" source="node5" target="node8">
      <mxGeometry relative="1" as="geometry" />
      <mxPoint x="550" y="420" as="sourcePoint" />
      <mxPoint x="560" y="490" as="targetPoint" />
    </mxCell>
    <mxCell id="edge7style" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#b85450;" edge="1" parent="1" source="node5" target="node8">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>

    
    <mxCell style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=1;exitY=0.5;exitDx=0;exitDy=0;entryX=0;entryY=0.5;entryDx=0;entryDy=0;" id="edge8" edge="1" parent="1" source="node6" target="node8">
      <mxGeometry relative="1" as="geometry" />
      <mxPoint x="900" y="420" as="sourcePoint" />
      <mxPoint x="810" y="490" as="targetPoint" />
    </mxCell>
    <mxCell id="edge8style" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=1;exitY=0.5;exitDx=0;exitDy=0;entryX=0;entryY=0.5;entryDx=0;entryDy=0;strokeColor=#b85450;" edge="1" parent="1" source="node6" target="node8">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>

    
    <mxCell style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0;exitY=0.5;exitDx=0;exitDy=0;entryX=0;entryY=0.5;entryDx=0;entryDy=0;dashed=1;dashed=1;dashed=1;dashed=1;" id="edge9" edge="1" parent="1" source="node7" target="node8">
      <mxGeometry relative="1" as="geometry" />
      <mxPoint x="1250" y="420" as="sourcePoint" />
      <mxPoint x="1060" y="490" as="targetPoint" />
    </mxCell>
    <mxCell id="edge9style" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0;exitY=0.5;exitDx=0;exitDy=0;entryX=0;entryY=0.5;entryDx=0;entryDy=0;dashed=1;dashed=1;dashed=1;dashed=1;strokeColor=#b85450;" edge="1" parent="1" source="node7" target="node8">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>

  </root>
</mxGraphModel></diagram></mxfile>