<mxfile host="app.diagrams.net"><diagram name="roadmap"><mxGraphModel dx="1200" dy="800" grid="1" gridSize="10" guides="1" tooltips="1" connect="1" arrows="1" fold="1" page="1" pageScale="1" pageWidth="1200" pageHeight="800" math="0" shadow="0">
<root>
<mxCell id="0" />
<mxCell id="1" parent="0" />
<mxCell id="node1" value="研究目标：解明雷公藤甲素P450合成酶系" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#dae8fc;strokeColor=#6c8ebf;fontSize=12;fontStyle=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
<mxGeometry x="218" y="40" width="278" height="64" as="geometry" />
</mxCell>
<mxCell id="edge1" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;" edge="1" parent="1" source="node1" target="node2">
<mxGeometry relative="1" as="geometry" />
</mxCell>
<mxCell id="node2" value="策略：转录组筛选68个候选TwCYP" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#fff2cc;strokeColor=#d6b656;fontSize=12;verticalAlign=middle;align=center;" vertex="1" parent="1">
<mxGeometry x="54" y="214" width="229" height="64" as="geometry" />
</mxCell>
<mxCell id="edge2" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;" edge="1" parent="1" source="node2" target="node3">
<mxGeometry relative="1" as="geometry" />
</mxCell>
<mxCell id="node3" value="双异源体系：本氏烟草+酵母工程" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#fff2cc;strokeColor=#d6b656;fontSize=12;verticalAlign=middle;align=center;" vertex="1" parent="1">
<mxGeometry x="40" y="388" width="229" height="64" as="geometry" />
</mxCell>
<mxCell id="edge3" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0;exitY=0.5;exitDx=0;exitDy=0;entryX=0;entryY=0.5;entryDx=0;entryDy=0;dashed=1;" edge="1" parent="1" source="node3" target="node4">
<mxGeometry relative="1" as="geometry" />
</mxCell>
<mxCell id="node4" value="关键改造1：CYP71BE86催化18(4→3)甲基迁移" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#f8cecc;strokeColor=#b85450;fontSize=12;verticalAlign=middle;align=center;" vertex="1" parent="1">
<mxGeometry x="353" y="214" width="306" height="64" as="geometry" />
</mxCell>
<mxCell id="edge4" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;" edge="1" parent="1" source="node4" target="node5">
<mxGeometry relative="1" as="geometry" />
</mxCell>
<mxCell id="node5" value="关键改造2：CYP71BE85+82D274+82D213完成环氧化" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#f8cecc;strokeColor=#b85450;fontSize=12;verticalAlign=middle;align=center;" vertex="1" parent="1">
<mxGeometry x="339" y="388" width="334" height="64" as="geometry" />
</mxCell>
<mxCell id="edge5" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;" edge="1" parent="1" source="node5" target="node6">
<mxGeometry relative="1" as="geometry" />
</mxCell>
<mxCell id="node6" value="关键改造3：共表达Twcytb5-A产量提升9倍" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#f8cecc;strokeColor=#b85450;fontSize=12;verticalAlign=middle;align=center;" vertex="1" parent="1">
<mxGeometry x="214" y="562" width="285" height="64" as="geometry" />
</mxCell>
<mxCell id="edge6" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;" edge="1" parent="1" source="node6" target="node7">
<mxGeometry relative="1" as="geometry" />
</mxCell>
<mxCell id="node7" value="结果：1L补料发酵产triptonide 3.79 mg/L" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#d5e8d4;strokeColor=#82b366;fontSize=12;fontStyle=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
<mxGeometry x="210" y="736" width="292" height="64" as="geometry" />
</mxCell>
<mxCell id="edge7" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;" edge="1" parent="1" source="node7" target="node8">
<mxGeometry relative="1" as="geometry" />
</mxCell>
<mxCell id="node8" value="结论：4个CYP构成最小基因集，验证发酵生产平台" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#d5e8d4;strokeColor=#82b366;fontSize=12;fontStyle=1;verticalAlign=middle;align=center;" vertex="1" parent="1">
<mxGeometry x="190" y="910" width="334" height="64" as="geometry" />
</mxCell>
<mxCell id="edge8" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;" edge="1" parent="1" source="node1" target="node4">
<mxGeometry relative="1" as="geometry" />
</mxCell>
</root>
</mxGraphModel></diagram></mxfile>