<mxfile host="app.diagrams.net"><diagram name="roadmap"><mxGraphModel dx="1200" dy="800" grid="1" gridSize="10" guides="1" tooltips="1" connect="1" arrows="1" fold="1" page="1" pageScale="1" pageWidth="1400" pageHeight="900" math="0" shadow="0">
<root>
<mxCell id="0" />
<mxCell id="1" parent="0" />
<mxCell id="node1" value="研究问题：酮类胡萝卜素来源有限、合成依赖石化、需可持续生物生产系统" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#dae8fc;strokeColor=#6c8ebf;fontSize=12;fontColor=#000000;verticalAlign=middle;align=center;" vertex="1" parent="1">
<mxGeometry x="634" y="40" width="340" height="64" as="geometry" />
</mxCell>
<mxCell id="node2" value="策略：选用海洋细菌crtW基因，组合玉米psy与泛菌crtI，农杆菌介导转化烟草BY-2细胞" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#d5e8d4;strokeColor=#82b366;fontSize=12;fontColor=#000000;verticalAlign=middle;align=center;" vertex="1" parent="1">
<mxGeometry x="634" y="162" width="340" height="64" as="geometry" />
</mxCell>
<mxCell id="node3" value="关键改造1：单独表达crtW基因（靶向质体，CaMV 35S驱动）" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#fff2cc;strokeColor=#d6b656;fontSize=12;fontColor=#000000;verticalAlign=middle;align=center;" vertex="1" parent="1">
<mxGeometry x="430" y="284" width="340" height="64" as="geometry" />
</mxCell>
<mxCell id="node4" value="关键改造2：共表达psy+crtI+crtW（组合代谢工程）" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#fff2cc;strokeColor=#d6b656;fontSize=12;fontColor=#000000;verticalAlign=middle;align=center;" vertex="1" parent="1">
<mxGeometry x="840" y="284" width="340" height="64" as="geometry" />
</mxCell>
<mxCell id="node5" value="结果1：单基因表达虾青素最高127 μg/g DW，角黄素55.1 μg/g DW" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#f8cecc;strokeColor=#b85450;fontSize=12;fontColor=#000000;verticalAlign=middle;align=center;" vertex="1" parent="1">
<mxGeometry x="40" y="406" width="340" height="64" as="geometry" />
</mxCell>
<mxCell id="node6" value="结果2：共表达YIW135角黄素788 μg/g DW（提升39倍）；YW02虾青素171.3 μg/g DW" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#f8cecc;strokeColor=#b85450;fontSize=12;fontColor=#000000;verticalAlign=middle;align=center;" vertex="1" parent="1">
<mxGeometry x="1229" y="406" width="340" height="64" as="geometry" />
</mxCell>
<mxCell id="node7" value="结果3：β-胡萝卜素含量显著提升，Y07达5035.8 μg/g DW（约60倍）" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#f8cecc;strokeColor=#b85450;fontSize=12;fontColor=#000000;verticalAlign=middle;align=center;" vertex="1" parent="1">
<mxGeometry x="450" y="406" width="340" height="64" as="geometry" />
</mxCell>
<mxCell id="node8" value="结果4：所有细胞系生长正常，色素稳定积累" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#f8cecc;strokeColor=#b85450;fontSize=12;fontColor=#000000;verticalAlign=middle;align=center;" vertex="1" parent="1">
<mxGeometry x="860" y="406" width="299" height="64" as="geometry" />
</mxCell>
<mxCell id="node9" value="结论：成功建立可扩展、可持续的酮类胡萝卜素植物细胞生物工厂，替代天然提取与化学合成" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#e1d5e7;strokeColor=#9673a6;fontSize=12;fontColor=#000000;verticalAlign=middle;align=center;" vertex="1" parent="1">
<mxGeometry x="634" y="528" width="340" height="64" as="geometry" />
</mxCell>
<mxCell id="edge1" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#6c8ebf;" edge="1" parent="1" source="node1" target="node2">
<mxGeometry relative="1" as="geometry" />
</mxCell>
<mxCell id="edge2" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#82b366;" edge="1" parent="1" source="node2" target="node3">
<mxGeometry relative="1" as="geometry" />
</mxCell>
<mxCell id="edge3" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#82b366;" edge="1" parent="1" source="node2" target="node4">
<mxGeometry relative="1" as="geometry" />
</mxCell>
<mxCell id="edge4" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#d6b656;" edge="1" parent="1" source="node3" target="node5">
<mxGeometry relative="1" as="geometry" />
</mxCell>
<mxCell id="edge5" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#d6b656;" edge="1" parent="1" source="node4" target="node6">
<mxGeometry relative="1" as="geometry" />
</mxCell>
<mxCell id="edge6" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#b85450;" edge="1" parent="1" source="node3" target="node7">
<mxGeometry relative="1" as="geometry" />
</mxCell>
<mxCell id="edge7" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#b85450;" edge="1" parent="1" source="node3" target="node8">
<mxGeometry relative="1" as="geometry" />
</mxCell>
<mxCell id="edge8" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#9673a6;" edge="1" parent="1" source="node5" target="node9">
<mxGeometry relative="1" as="geometry" />
</mxCell>
<mxCell id="edge9" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#9673a6;" edge="1" parent="1" source="node6" target="node9">
<mxGeometry relative="1" as="geometry" />
</mxCell>
<mxCell id="edge10" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#9673a6;" edge="1" parent="1" source="node7" target="node9">
<mxGeometry relative="1" as="geometry" />
</mxCell>
<mxCell id="edge11" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#9673a6;" edge="1" parent="1" source="node8" target="node9">
<mxGeometry relative="1" as="geometry" />
</mxCell>
</root>
</mxGraphModel></diagram></mxfile>