<mxfile host="app.diagrams.net"><diagram name="roadmap"><mxGraphModel dx="1200" dy="800" grid="1" gridSize="10" guides="1" tooltips="1" connect="1" arrows="1" fold="1" page="1" pageScale="1" pageWidth="1400" pageHeight="900" math="0" shadow="0">
  <root>
    <mxCell id="0" />
    <mxCell id="1" parent="0" />
    <mxCell id="n1" value="核心问题：改善大豆制品营养价值，解析增强棉子糖代谢菌株的分解代谢机制" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#dae8fc;strokeColor=#6c8ebf;fontSize=12;fontStyle=1;horizontal=1;verticalAlign=middle;align=center;spacing=5;arcSize=8;" vertex="1" parent="1">
      <mxGeometry x="40" y="40" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="n2" value="研究策略：利用iTRAQ标记比较蛋白质组学分析工程菌株与亲本菌株" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#d5e8d4;strokeColor=#82b366;fontSize=12;fontStyle=0;horizontal=1;verticalAlign=middle;align=center;spacing=5;arcSize=8;" vertex="1" parent="1">
      <mxGeometry x="40" y="162" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="n3" value="核心改造：过表达α-半乳糖苷酶（LBP_cg2832）的工程化L. plantarum P-8" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#ffe6cc;strokeColor=#d79b00;fontSize=12;fontStyle=0;horizontal=1;verticalAlign=middle;align=center;spacing=5;arcSize=8;" vertex="1" parent="1">
      <mxGeometry x="40" y="284" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="n4" value="关键发现1：生长性能提升与碳源代谢切换（棉子糖→山梨醇）" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#f8cecc;strokeColor=#b85450;fontSize=12;fontStyle=0;horizontal=1;verticalAlign=middle;align=center;spacing=5;arcSize=8;" vertex="1" parent="1">
      <mxGeometry x="40" y="406" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="n5" value="关键发现2：山梨醇PTS系统及脱氢酶显著上调（EIIA 5.8倍，脱氢酶10.6倍）" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#f8cecc;strokeColor=#b85450;fontSize=12;fontStyle=0;horizontal=1;verticalAlign=middle;align=center;spacing=5;arcSize=8;" vertex="1" parent="1">
      <mxGeometry x="40" y="528" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="n6" value="关键发现3：酸胁迫适应机制（膜脂修饰+氨基酸代谢调控）" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#f8cecc;strokeColor=#b85450;fontSize=12;fontStyle=0;horizontal=1;verticalAlign=middle;align=center;spacing=5;arcSize=8;" vertex="1" parent="1">
      <mxGeometry x="40" y="650" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="n7" value="结论：工程菌株通过替代碳源利用和膜/氨基酸代谢重塑适应酸性环境" style="rounded=1;whiteSpace=wrap;html=1;fillColor=#fff2cc;strokeColor=#d6b656;fontSize=12;fontStyle=1;horizontal=1;verticalAlign=middle;align=center;spacing=5;arcSize=8;" vertex="1" parent="1">
      <mxGeometry x="40" y="772" width="340" height="64" as="geometry" />
    </mxCell>
    <mxCell id="e1" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#6c8ebf;" edge="1" parent="1" source="n1" target="n2">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="e2" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#6c8ebf;" edge="1" parent="1" source="n2" target="n3">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="e3" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#6c8ebf;" edge="1" parent="1" source="n3" target="n4">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="e4" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#6c8ebf;" edge="1" parent="1" source="n4" target="n5">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="e5" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#6c8ebf;" edge="1" parent="1" source="n5" target="n6">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
    <mxCell id="e6" style="edgeStyle=orthogonalEdgeStyle;rounded=1;orthogonalLoop=1;jettySize=auto;html=1;endArrow=block;endFill=1;strokeWidth=1.6;exitX=0.5;exitY=1;exitDx=0;exitDy=0;entryX=0.5;entryY=0;entryDx=0;entryDy=0;strokeColor=#6c8ebf;" edge="1" parent="1" source="n6" target="n7">
      <mxGeometry relative="1" as="geometry" />
    </mxCell>
  </root>
</mxGraphModel></diagram></mxfile>